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DAF-2 DA
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Catalog No. T18966Cas No. 205391-02-2
Alias DAF-2DA, DAF2DA, DAF 2DA, 5,6-Diaminofluorescein diacetate
DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm.
DAF-2 DA
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Catalog No. T18966Alias DAF-2DA, DAF2DA, DAF 2DA, 5,6-Diaminofluorescein diacetateCas No. 205391-02-2
DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $155 | 35 days | 35 days | |
| 250 μg | $347 | 35 days | 35 days | |
| 500 μg | $656 | 35 days | 35 days | |
| 1 mg | $1,237 | 35 days | 35 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Appearance:Liquid
Color:Transparent
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| Description | DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm. |
| In vitro | Steps for assessing total mitochondrial nitric oxide levels: 1. SH-SY5Y cells were seeded in duplicate at a density of 5 × 10^3 cells/well in black 96-well cell culture plates. 2. After gelsemium treatment, cells were incubated with DAF-2 DA (10 μM, 15 minutes) on an orbital shaker at room temperature in the dark. 3. After washing twice with HBSS (Sigma) using a Cytation 3 cell imaging multi-function microplate reader, the formation of a green fluorescent product was detected at 485 nm (excitation)/538 nm (emission). The fluorescence intensity is the total cellular nitric oxide level. [2] |
| Synonyms | DAF-2DA, DAF2DA, DAF 2DA, 5,6-Diaminofluorescein diacetate |
| Molecular Weight | 446.41 |
| Formula | C24H18N2O7 |
| Cas No. | 205391-02-2 |
| Smiles | O=C1OC2(C3=CC=C(OC(=O)C)C=C3OC4=CC(OC(=O)C)=CC=C42)C5=CC(N)=C(N)C=C15 |
| Relative Density. | 1.54g/cm3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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