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LysoTracker Yellow HCK 123

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Catalog No. T86840Cas No. 1064123-31-4

LysoTracker Yellow HCK 123 is a highly selective fluorescent probe designed for labeling acidic organelles in live cells, particularly lysosomes. The probe accumulates in acidic compartments via its weak base moiety. In plant sciences, it has been discovered to specifically label silica-deposition vesicles (SDVs) in diatoms, serving as a vital tool for studying biosilicification. In pharmacological research, it is frequently utilized to track the intracellular localization of drug delivery systems (e.g., polymeric micelles) to verify endocytosis and trafficking pathways through colocalization analysis.

LysoTracker Yellow HCK 123

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Purity: 98.89%

Catalog No. T86840Cas No. 1064123-31-4

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$116	-	In Stock
5 mg	$197	-	In Stock
10 mg	$322	-	In Stock
25 mg	$538	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.89%

Appearance:Solid

Color:Orange

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description

In vitro

In cellular assays, LysoTracker Yellow HCK 123 is typically applied at a final concentration of 0.1-50 µM. After incubating cells (e.g., in 8-well slides) at 37 °C and 5% CO2 for 30 minutes, followed by a PBS wash, the samples are analyzed via confocal laser scanning microscopy (CLSM) to effectively visualize acidic organelles and track endocytic pathways [1][2].

Chemical Properties

Molecular Weight	364.4
Formula	C₁₆H₂₄N₆O₄
Cas No.	1064123-31-4
Smiles	O=C(CCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C21)NCCN(C)C

Storage & Solubility Information

Storage

keep away from direct sunlight | store at -20°C | Shipping with blue ice.

Solubility Information

DMSO: 50 mg/mL (137.21 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7442 mL	13.7212 mL	27.4424 mL	137.2119 mL
5 mM	0.5488 mL	2.7442 mL	5.4885 mL	27.4424 mL
10 mM	0.2744 mL	1.3721 mL	2.7442 mL	13.7212 mL
20 mM	0.1372 mL	0.6861 mL	1.3721 mL	6.8606 mL
50 mM	0.0549 mL	0.2744 mL	0.5488 mL	2.7442 mL
100 mM	0.0274 mL	0.1372 mL	0.2744 mL	1.3721 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc