1m-7

1M6 (1-Methyl-6-nitroisatoic anhydride) is a SHAPE reagent for the detection of RNA chemical structures.

1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent. It detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.

7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) serves as a fluorescent probe for detecting hydrogen sulfide (H2S). It is characterized by a low detection limit (4×10^-6 mol/L), high selectivity, and great sensitivity. At a concentration of 150 µg/mL, it exhibits negligible cytotoxicity. The excitation wavelength is 331 nm, while in DMSO solvent, it emits at approximately 385 nm; upon addition of HS-, the fluorescence intensity significantly increases around 392 nm.

PRMT5-targeted fluorescent ligand-1 (Compound 7) is a fluorescent ligand specific to PRMT5 that inhibits this target with an IC50 value of 29.39 nM. It demonstrates cellular imaging activity with strong fluorescence signals in MCF-7 cells, showing an IC50 of 29 nM. Additionally, it induces apoptosis (cell death). The compound's maximum excitation wavelength is 438 nm, and its maximum emission wavelength is 550 nm.

8-(4-ethynylphenyl)-1,3,5,7-tetramethyl BODIPY is an organic fluorescent dye characterized by an ethynylphenyl group at the 8-position of the boron dipyrromethene (BODIPY) structure and methyl substituents at the 1, 3, 5, and 7 positions. It boasts a high fluorescence quantum yield and specific chemical properties, making it useful for applications such as fluorescence imaging.

8-(4-bromophenyl)-1,3,5,7-tetramethyl-BODIPY is a derivative based on the BODIPY structure, featuring four methyl groups at positions 1, 3, 5, and 7, with a para-bromophenyl group attached at position 8. This compound finds extensive use in fields such as bioimaging, chemical analysis, and materials science, serving as a fluorescent probe for imaging biological samples, detecting specific chemicals, or in the development of optical materials.

Biotin-Glucagon-Like Peptide 1 (7-36) amide, human, is an active peptide useful in various biochemical studies.

Ac-Orn-Phe-Arg-AMC is an effective fluorescent substrate for cd-Tryptase and β1-Tryptase. This compound releases 7-amino-4-methylcoumarin (AMC), which is utilized for fluorescence detection.

FD dye 7 (compound 7) serves as an intermediate for cyanine-based fluorescent dyes, facilitating the synthesis of dyes FD-1 and FD-2.

STY-BODIPY (Styrene-BODIPY) is a styrene-conjugated fluorescent probe enabling calculation of radical trapping antioxidant (RTA) activity by monitoring absorbance loss at 571 nm.

7-Azido-4-methylcoumarin (7-azido-4-methyl-2H-chromen-2-one) is a fluorescent photoactive probe used in the study of human phenol sulfotransferase (SULT1A1 or P-PST-1) substrate binding sites through photoaffinity labeling.

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation/emission maxima of 380/515 nm, respectively.1 1.Imai, K., Toyo'oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

Ac-ANW-AMC is a fluorogenic substrate for the β5i/LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released, and its fluorescence quantifies the activity of the β5i/LMP7 subunit. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

7ACC1 (D 142) selectively affects a single part of the MCT symporter translocation cycle, leading to strict inhibition of lactate influx. This singular activity is associated with antitumor effects less prone to resistance and side effects.

7-Dimethylaminocoumarin-4-acetic acid, catalog number T64950, CAS number 80883-54-1, is a valuable organic compound for life sciences research.

7-(Diethylamino)-4-methyl-2H-chromen-2-one is a useful organic compound for research related to life sciences. The catalog number is T66262 and the CAS number is 91-44-1.

4,6-Dimethyl-7-ethylaminocoumarin is a valuable organic compound for life sciences research (Catalog number: T67631, CAS number: 26078-25-1).

7-Hydroxy-4-methyl-2(1H)-quinolone (compound 2b), a fluorescent hydroxylated derivative, serves as a probe for detecting hydroxyl radicals associated with DNA damage [1]. It should be protected from light for preservation.

Mca-(Ala7,Lys(Dnp)9)-bradykinin is a fluorogenic substrate that demonstrates high sensitivity for ECE-1 (endothelin-converting enzyme-1). It features a (7-methoxycoumarin-4-yl)acetyl (Mca) group for fluorescence and a 2,4-dinitrophenyl (Dnp) group for quenching, yielding a significant increase in fluorescence when the substrate is cleaved [1].

Sulfo-Cy3-Methyltetrazine (figure 7 compound 5), a click chemistry reagent, comprises methyltetrazine linked to a water-soluble dye. This compound demonstrates good stability at physiological pH and can efficiently couple to trans-cyclooctene, showing high reactivity towards cyclooctene [1].

Mca-SEVNLDAEFK(Dnp)-NH2 is a fluorogenic substrate featuring a 7-methoxycoumarin moiety whose fluorescence is quenched by the adjacent 2,4-dinitrophenyl group through resonance energy transfer. This compound serves as a probe for assessing peptidase activity; enzymatic cleavage of the amide bond separating the fluorophore and quencher results in increased fluorescence. It is particularly useful for quantifying the activity of BACE-1 [1].