Anti-ZO-1/TJP1 Antibody (1M732) is a Rabbit antibody targeting ZO-1/TJP1. Anti-ZO-1/TJP1 Antibody (1M732) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P.

IgG

1M732

Human,Mouse,Rat

1. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (ZO-1) monoclonal Antibody, unconjugated (TMAB-14458) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei.
7. The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (TMAB-14458, 1 : 100）; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
12. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ZO-1/TJP1 Monoclonal Antibody, Unconjugated (TMAB-14458) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

FCM=1 μg/Test; ICC/IF=1:100-200; IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500

Monoclonal

Recombinant Protein: human ZO-1 protein

Human

TJP1

Tight junction protein ZO-1

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.