mannosidase

Swainsonine (Tridolgosir) is an alkaloid isolated from Astragalus membranaceus and is a potent and reversible inhibitor of alpha-mannosidase. swainsonine has antitumour activity and induces apoptosis and cell cycle arrest in the G2/M phase.

exo-α-1,2-Mannosidase, Bacteroides thetaiotaomicron (EC.3.2.1.163), is a glycoside hydrolase involved in the breakdown of mannans.

exo-α-Mannosidase, Bacteroides thetaiotaomicron (EC.3.2.1.24), is a glycoside hydrolase involved in the breakdown of mannans.

Exo-α-1,4-Mannosidase, Bacteroides thetaiotaomicron (EC.3.2.1.163), is a glycoside hydrolase involved in the degradation of mannan.

Exo-α-1,3-Mannosidase, derived from Bacteroides thetaiotaomicron, is a glycoside hydrolase enzyme that specifically targets and hydrolyzes the mannose α-1,3 linkages present in host N-glycans.

Exo-α-1,6-Mannosidase, Bacteroides thetaiotaomicron (EC 3.2.1.163), is a glycoside hydrolase that specifically hydrolyzes the mannose α-1,6 linkages in host N-glycans.

β-Mannosidase, Bacteroides fragilis (EC 3.2.1.25), is the final exoglycosidase in the catabolic pathway of N-linked glycoprotein oligosaccharides. It catalyzes the hydrolysis of terminal non-reducing β-D-mannosyl residues in β-D-mannosides.

β-Mannosidase, Bacteroides thetaiotaomicron (EC 3.2.1.25), serves as the final exoglycosidase in the catabolic pathway of N-linked glycoprotein oligosaccharides. It catalyzes the hydrolysis of terminal non-reducing β-D-mannosyl residues in β-D-mannosides.

α1-2,3 Mannosidase, Xanthomonas manihotis, is an acidic hydrolase found within plant vacuoles and is believed to play a role in the turnover of N-linked glycoproteins.

β-Mannosidase, Cellvibrio mixtus (EC 3.2.1.25), is the terminal exoglycosidase in the catabolic pathway of N-linked glycoprotein oligosaccharides. This enzyme catalyzes the hydrolysis of terminal, non-reducing β-D-mannose residues in β-D-mannosides.

β-Mannosidase, Helix pomatia (EC 3.2.1.25) catalyzes the hydrolysis of terminal non-reducing β-D-mannosyl residues in β-D-mannosides. It is the final exoglycosidase in the catabolic pathway of N-linked glycoprotein oligosaccharides.

1,6-α-D-Mannosidase (EC 3.2.1.163) specifically catalyzes mannobiose linked through (1→6) bonds and exhibits no activity towards other linkage types, p-nitrophenyl-α-D-mannopyranoside, or baker's yeast mannan. The enzyme's activity is strongly inhibited by Mn2+ but does not require Ca2+ or other metal cofactors for its function.

β-1,2-Mannosidase (EC 3.2.1.197) has been isolated and identified from various bacteria and is capable of catalyzing the hydrolysis of non-reducing terminal D-mannose residues in β-1,2-mannobiose and β-1,2-mannotriose. Its mechanism involves anomeric inversion, ultimately releasing α-D-mannopyranose. This enzyme exhibits higher activity on β-1,2-mannotriose or longer oligosaccharides compared to its activity on β-1,2-mannobiose.

β-Mannosidase from Streptomyces coelicolor (EC 3.2.1.25) catalyzes the hydrolysis of terminal, non-reducing β-D-mannose residues in β-D-mannosides. This gene encodes a member of the glycoside hydrolase family 2. The encoded protein is localized in the lysosome and acts as the final exoglycosidase in the catabolic pathway of N-linked glycoprotein oligosaccharides. Mutations in this gene are associated with β-mannosidosis, a lysosomal storage disorder affecting several neurological systems.

α-Mannosidase is a biochemical reagent used as a biological material or organic compound in life science research.

Lysosomal α-mannosidase (EC:3.2.1.24; MAN2B1; LAMAN) is an exoglycosidase that hydrolyzes the α-mannosidic linkages in mannose-containing glycans. It is utilized in research related to α-mannosidosis.

1-Deoxymannojirimycin hydrochloride, a selective α1,2-mannosidase inhibitor (IC50: 20 μM), also inhibits HIV-1 strains but exhibits poor antiviral activity.

exo-α-1,2/3-Arabinofuranosidase, Penicillium oxalicum (EC.3.2.1.77), is a glycoside hydrolase enzyme that hydrolyzes the α-1,2 and α-1,3 mannose linkages in host N-glycans.

Plantagoside is a specific non-competitive inhibitor for jack bean alpha-mannosidase (IC50: 5 μM). It also is a potent inhibitor of the Maillard reaction.

D-(-)-Quinic acid (Kinic acid) is a cellular metal ion chelator, capable of promoting reactions with metal M(II,III) ions under pH-specific conditions. It may possess potent inhibition against alpha-mannosidase and alpha-fucosidase.

Kifunensine, an alkaloid discovered in Kitasatosporia kifunense, functions as a glycosylation pathway inhibitor and immunomodulator. It strongly inhibits α-mannosidase I in the Golgi apparatus and may be utilised in studies of endoplasmic reticulum stress.

2DG-ODDA is a potent antitumor derivative of 2-deoxyglucose (2-DG). It induces apoptosis and reduces ATP production. 2DG-ODDA is absorbed via fatty acid and glucose transporters and cleaved by α-Mannosidase to release 2DG, thereby inhibiting N-glycosylation and disrupting cellular metabolism. It suppresses tumor growth in 4T1 murine models and is applicable in triple-negative breast cancer (TNBC) research.

OMJ-4 is a modulator of mutant α-mannosidase (alpha-mannosidase). It restores the catalytic function of the mutant α-mannosidase and enhances substrate binding capability by altering its compactness and active site flexibility. Acting as a substrate reactivity inducer, OMJ-4 increases the reactivity of mannose disaccharides binding to mutant α-mannosidase and lowers the activation energy required for the enzyme-catalyzed reaction. OMJ-4 is useful for research related to α-mannosidosis.

Australine is a pyrrolizidine alkaloid originally isolated fromC. australethat has enzyme inhibitory activities.1,2,3It is an inhibitor of glucoamylase (IC50= 5.8 μM) that also inhibits glucosidase I, sucrase, maltase, andA. nigerα-glucosidase (IC50s = 20, 28, 35, and 28 μM, respectively).2,3Australine is selective for these enzymes over glucosidase II, α- and β-mannosidase, and α- and β-galactosidase up to 500 μM, β-glucosidase, with only 5% inhibition at 66 μM, as well as isomaltase and trehalase (IC50= 97 and 160 μM, respectively). Australine (500 μg/ml) inhibits glycoprotein processing of viral glycoproteins in influenza virus-infected MDCK cells and induces the accumulation of glycoproteins.2 1.Molyneux, R.J., Benson, M., Wong, R.Y., et al.Australine, a novel pyrrolizidine alkaloid glucosidase inhibitor from Castanospermum australJ. Nat. Prod.51(6)1198-1206(1988) 2.Tropea, J.E., Molyneux, R.J., Kaushal, G.P., et al.Australine, a pyrrolizidine alkaloid that inhibits amyloglucosidase and glycoprotein processingBiochemistry28(5)2027-2034(1989) 3.Kato, A., Kano, E., Adachi, I., et al.Australine and related alkaloids: easy structural confirmation by 13C NMR spectral data and biological activitiesTetrahedron Asymmetry14(3)325-331(2003)