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Flow cytometry

Flow cytometry dyes are a class of fluorescent markers used in flow cytometric analysis, serving as essential tools in research fields such as immunophenotyping, cellular function studies, and signaling pathway investigations.

Alexa Fluor 430 NHS ester
T203214
Alexa Fluor 430 NHS ester is a compound used to conjugate the green fluorescent dye Alexa Fluor 430 with proteins or antibodies. Alexa Fluor 430 provides stable signal generation in imaging and flow cytometry. The NHS ester is suitable for labeling proteins, amine-modified oligonucleotides, and other primary amine-containing molecules (R-NH2).
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6-FITC
6-Fluorescein Isothiocyanate
T140583326-31-6
6-FITC is a commonly used green fluorescent labeling dye and a fluorescein isothiocyanate derivative with excitation and emission wavelengths of 495 nm and 520 nm, respectively. 6-FITC can covalently bind to amino groups in proteins, antibodies, or peptides, making it suitable for a variety of applications, including flow cytometry, fluorescence microscopy, and tissue section staining. Since FITC itself is not cell-permeable, it is typically used to label surface antigens on live cells; however, it can also be used for intracellular protein detection after cell fixation or permeabilization.
  • $30
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Sulfo-Cy3 NHS ester TEA
Sulfo-Cy3 NHS ester TEA (146368-16-3 free base)
T18943L
Sulfo-Cy3 NHS ester TEA (146368-16-3 free base) is a sulfonated cyanine-based fluorescent labeling reagent with an excitation wavelength of approximately 550 nm and an emission wavelength of approximately 570 nm. Sulfo-Cy3 NHS ester TEA can be used as an amine-reactive fluorescent probe for labeling biomolecules such as antibodies, proteins, peptides, and nucleic acid probes. Sulfo-Cy3 NHS ester TEA is also suitable for applications including immunofluorescence staining, in situ hybridization, and flow cytometry analysis.
  • $195
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AF647 Me-tetrazine
Alexa Fluor 647 Me-tetrazine
T212874
AF647 Me-tetrazine is a multifunctional fluorescent probe formed by the covalent coupling of the far-red fluorescent dye Alexa Fluor 647 (AF647) with a methyl tetrazine (Me-tetrazine) group (Ex/Em ≈ 647/668 nm). AF647 Me-tetrazine is widely used in research applications such as multicolor fluorescence imaging of live cells and living organisms, flow cytometry, and single-molecule tracking.
  • $926
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Geldanamycin-FITC
Geldanamycin FITC
T740132969156-01-0
Geldanamycin-FITC is a fluorescence probe based on geldanamycin and is suitable for fluorescence polarization analysis of HSP90 inhibitors. In addition, Geldanamycin-FITC can also be used to detect cell-surface HSP90.
  • $1,498
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SBB-Analogue (GL13) Biotin
Sudan Black B-Analogue (GL13) Biotin, SBB-A-B, GL13
T893962171036-89-6
The compound SBB-Analogue (GL13) Biotin (SBB-A-B) is formed by linking a derivative of Sudan Black B (SBB) with biotin. It effectively detects senescent cells, avoids false-positive SA-β-gal results caused by serum starvation or cell fusion, and is compatible with experimental techniques such as flow cytometry and immunofluorescence analysis.
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AF594 DBCO
T203579
AF594 DBCO is a fluorescent labeling reagent that combines the red fluorescent dye AF594 with DBCO (dibenzocyloctyne). DBCO is a frequently used chemical biolabeling group. AF594 DBCO (excitation wavelength about 590 nm, emission wavelength about 617 nm) is suitable for labeling proteins, cells, and other biomolecules for fluorescence imaging and flow cytometry detection.
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eGFP mRNA-LNP (500ng/uL)
T203248
eGFP mRNA-LNP (500 ng/uL) is a lipid nanoparticle (LNP) containing eGFP mRNA, suitable for assays of RNA delivery, translation efficiency, and cell viability. eGFP circRNA includes the Enhanced Green Fluorescent Protein (eGFP), which expresses green fluorescent protein upon entering cells. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry.
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HKOH-1r
T396002138472-08-7
HKOH-1r is a highly sensitive and selective green fluorescent probe for detecting endogenous hydroxyl radicals (·OH) in live cells, with maximum excitation and emission wavelengths at 500 nm and 520 nm, respectively. Within a concentration range of 0.1–50 μM, HKOH-1r exhibits low cytotoxicity toward RAW264.7 and HeLa cells. It has been used on flow cytometry platforms to detect intracellular endogenous ·OH levels and is suitable for research on reactive oxygen species (ROS), oxidative stress, and related cellular damage mechanisms.
  • $747
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