droplets

Nile Red ((Nile Blue A oxazone)) is a lipophilic, environment-sensitive fluorescent dye. Highly hydrophobic, with fluorescence significantly enhanced in lipid-rich environments. Commonly used for specific staining of intracellular lipid droplets, with excitation/emission wavelengths of 559/635 nm.

Lyso BD-1 predominantly colocalizes with lysosomes and lipid droplets, exhibiting exceptional photocytotoxicity (5.57 μM) against triple-negative breast cancer cells under white light irradiation. The emission wavelength of Lyso BD-1 is 560 nm.

PTZ-LD, a phenothiazine-based fluorescent probe, specifically detects lipid droplets (LDs) with apparent emissivity (Ex/Em=488/570-620 nm) and is utilized in diabetic cataract (DC) research [1].

BETA-1 is the first integrated molecule combining intramolecular charge transfer and aggregation-induced emission. It can generate cyan and red fluorescent signals in lipid droplets and mitochondria, respectively, and is suitable for dual-color simultaneous imaging of lipid droplets and mitochondria in both in vivo and in vitro systems.

2,6-Diiodo-BODIPY 493/503 is a modified BODIPY fluorescent dye whose 2,6-diiodo substituent enhances its photostability and resistance to photobleaching. 2,6-Diiodo-BODIPY 493/503 is commonly used for labeling and imaging lipid droplets and lipid-associated structures.

BODIPY 500/510 C1, C12, a derivative of the robust BODIPY dye family, exhibits strong ultraviolet absorption and emits fluorescence with a high quantum yield at a sharp peak. This dye's effectiveness is minimally affected by environmental factors such as polarity or pH, and it maintains stability under various physiological conditions. BODIPY 500/510 C1, C12 has the unique ability to penetrate cell membranes and specifically target polar lipids within cells, effectively staining lipid droplets. This property makes it suitable for labeling both live and fixed cells [1]. It has a maximum excitation/emission wavelength of 500/510 nm [1]. For optimal preservation, it should be protected from light and stored at -20°C.

BODIPY 505/515-8-C3-COOH, a green fluorescing derivative, serves as a fluorescent dye, particularly useful in imaging lipid droplets in nannochloropsis. It is also applicable in flow cytometric high-throughput screening and cell sorting research [1].

BODIPY493/503 methyl bromide, a BODIPY dye, is a small molecule with strong ultraviolet absorption, a sharp fluorescence peak, and high quantum yield. It exhibits relative insensitivity to environmental polarity and pH while maintaining stability under various physiological conditions. The structural asymmetry of BODIPY allows for diverse structural derivatives. BODIPY lipid droplet dyes effectively permeate the cell membrane, localize polar lipids, and specifically stain lipid droplets, making them suitable for labeling both live and fixed cells [1]. Maximum excitation/emission wavelength: 493/503 nm [1].

BODIPYFL C12 is a BODIPY dye, a small molecule dye with strong ultraviolet absorption ability, a sharp fluorescence peak, and high quantum yield. It is relatively insensitive to environmental polarity and pH and remains stable under different physiological conditions. Due to its structural asymmetry, BODIPY derivatives exhibit various structural products. BODIPY lipid droplet dyes effectively penetrate cell membranes, localize polar lipids, and specifically stain lipid droplets, making them suitable for labeling live and fixed cells [1]. Maximum excitation/emission wavelength: 480/508 nm [1].

Liptracker-green (fac-[Re(CO)3(phen)L]) serves as a cell-permeable fluorogenic probe specifically targeting polar lipids. It effectively labels lipid droplets and compartments rich in lipids within both live and fixed cells for analysis via fluorescent microscopy.

SMCy5.5, an SMCy dye for lipid droplet labeling, exhibits a very high two-photon absorption cross-section. Unlike the well-known Nile Red, SMCy has narrow absorption and emission bands in visible light, facilitating multicolor imaging. SMCy is compatible with fixation, yielding high-quality 3D images of lipid droplets in cells and tissues. The high brightness of SMCy5.5 enables efficient tracking and imaging of lipid droplet exchange between adipocytes [1].

ROX Reference Dye is an inert fluorescent dye used in real-time PCR (qPCR) or RT-PCR to normalize fluorescence signals between wells. It does not participate in the PCR reaction and maintains a stable fluorescence signal throughout the process。ROX dye can correct for variations in fluorescence signals caused by uneven lighting, differences in well optics (such as droplets, bubbles), and variations in reaction mixture concentration。