blotting

Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification.

Nitro blue tetrazolium chloride (NBT), an NADPH-diaphorase substrate (IC50 = 3-4 M) and well-known scavenger of superoxide anions, competitively inhibits nitric oxide synthase. Additionally, NBT, as a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Filricianine sodium is a hydrophilic fluorescent dye. It can bind to antibodies with a higher degree of labeling (D/P ratio) and is widely used in protein blotting, microscopy, flow cytometry, and cell-based assays.

BP Light 800 NHS ester is an amine-reactive hydrophilic fluorescent dye. It is capable of binding to antibodies with a higher D/P ratio and is widely used in applications such as protein blotting, microscopy, flow cytometry, and cell-based assays.

DY-680-NHS ester is an amine-reactive, hydrophilic fluorescent dye. It efficiently conjugates with antibodies at high degrees of labeling (D/P ratios) without causing fluorescence quenching or precipitating the conjugates. This compound is widely used in applications such as protein blotting, microscopy, flow cytometry, and cell-based assays. Its spectral properties include an excitation wavelength of 690 nm and an emission wavelength of 709 nm.