alkyne-probe 1

Alkyne-probe 1 is typically employed as an Alkyne-labeled fluorescent or chemical probe.

DIBA-Cy5, a fluorescent DIBA antagonist, consists of DIBA-alkyne and Cyanine5 fluorophores (Cy5) linked by a polyethylene glycol (PEG) chain. It functions as a fluorescent ligand for probe attachment via click chemistry, exhibiting high binding affinity to type-2 mAChR (M2R) with a dissociation constant (Kd) of 1.80 nM. Notably, DIBA-Cy5 effectively stains M2R receptors in the sinoatrial node of mouse hearts [1].

Chol-N3 is a bioorthogonal chol probe capable of integrating with super-resolution fluorescence microscopy (SRM) to enable direct nanoscale imaging of lipid heterogeneity on resting living cell surfaces [1]. As a click chemistry reagent with an azide group, Chol-N3 undergoes copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-bearing molecules and can also participate in strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO or BCN group-containing molecules.

Fl-DIBO (fluorogenic dibenzocyclooctyne) serves as a selective fluorescent probe with high sensitivity for azide compounds, rapidly forming highly fluorescent products upon reaction. These products showcase a maximum emission wavelength of 469 nm alongside an excitation wavelength of 363 nm. Fl-DIBO is particularly useful for labeling diazo-tagged proteins, maintaining clarity by avoiding detectable background signal interference [1] [2]. As a click chemistry reagent, Fl-DIBO contains an Alkyne group and is capable of undergoing copper-catalyzed azide-alkyne cycloaddition (CuAAc) with Azide-containing molecules.