thiazole orange

Thiazole Orange is a dye that can be used for reticulocyte analysis.

(Z)-Thiazole Orange iodide is an asymmetric cyanine dye with fluorescence highly dependent on its local environment. In solution, it is generally non-fluorescent; however, its fluorescence increases by a factor of one thousand when bound to double-stranded DNA and RNA (dsDNA or dsRNA). The compound's absorption maxima while complexed with DNA is 509 nm, and its emission maxima is 532 nm. (Z)-Thiazole Orange iodide solutions are widely utilized for determining the percentage of reticulocytes in human peripheral blood using microscopy and flow cytometry.

Thiazole orange dimer YOYO-1 is a green fluorescent dye used for DNA staining. It is a tetracationic homodimer of oxazole yellow (YO) and is typically provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) exhibits low fluorescence quantum yield, but upon binding to double-stranded DNA through diintercalation, its fluorescence intensity increases by 3200 times (λmax 489 nm; λmax 509 nm).