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Thiazole Orange

🥰Excellent
Catalog No. T19065Cas No. 107091-89-4

Thiazole Orange is a dye that can be used for reticulocyte analysis.

Thiazole Orange

Thiazole Orange

🥰Excellent
Purity: 99.93%
Catalog No. T19065Cas No. 107091-89-4
Thiazole Orange is a dye that can be used for reticulocyte analysis.
Pack SizePriceAvailabilityQuantity
25 mg$30In Stock
50 mg$39In Stock
1 mL x 10 mM (in DMSO)$61In Stock
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Purity:99.93%
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Product Introduction

Bioactivity
Description
Thiazole Orange is a dye that can be used for reticulocyte analysis.
Cell Research
Instructions
I. Solution preparation
1. Stock solution: Dissolve Thiazole Orange in DMSO or ethanol to prepare a stock solution with a concentration of 13 mg/ml. Since the dye is light-sensitive, it needs to be handled and stored in the dark.
2. Working solution: Dilute the stock solution to the working concentration according to the experimental requirements, usually 1 mg/ml. Use an appropriate buffer (such as PBS) for dilution.
II. Operation steps
1. Coupling with oligonucleotides:
(1) Thiazole Orange can be covalently coupled to modified oligonucleotides or linkers through amino-reactive functional groups (such as NHS esters or isothiocyanates).
(2) After coupling, purify the labeled oligonucleotides by high-performance liquid chromatography (HPLC) or gel electrophoresis.
(3) Verify the coupling efficiency by absorption and fluorescence measurements.
2. Reticulocyte analysis:
(1) Prepare a red blood cell suspension (prepared in PBS or isotonic buffer).
(2) Add Thiazole Orange at a working concentration (usually 1 mg/ml) and incubate at 37°C for 60 minutes to allow it to bind to RNA in reticulocytes.
(3) Analyze the stained cells using a flow cytometer or fluorescence microscope, selecting appropriate excitation wavelengths (~510 nm) and emission wavelengths (~530 nm).
3. Application of fluorescent hybridization probes:
(1) Use labeled oligonucleotide probes to detect specific nucleic acid sequences in hybridization experiments.
(2) Optimize hybridization conditions (such as temperature and buffer composition) to ensure specific binding of the probe to the target sequence.
(3) Measure the fluorescent signal using a fluorometer or fluorescence imaging system.
Notes:
1. Photosensitivity: Thiazole Orange is light-sensitive and should be avoided from prolonged exposure to strong light when used.
2. Storage conditions: The stock solution should be stored in a sealed container at -20°C to reduce moisture exposure and prevent degradation. Avoid repeated freezing and thawing.
3. Compatibility: Thiazole Orange binds strongly to nucleic acids. Avoid using solutions containing detergents or high salt concentrations, which may reduce its interaction with nucleic acids.
Chemical Properties
Molecular Weight476.61
FormulaC26H24N2O3S2
Cas No.107091-89-4
SmilesCc1ccc(cc1)S([O-])(=O)=O.CN1\C(Sc2ccccc12)=C\c1cc[n+](C)c2ccccc12
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: < 0.1 mg/mL (insoluble)
DMSO: 25 mg/mL (52.45 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.0982 mL10.4908 mL20.9815 mL104.9076 mL
5 mM0.4196 mL2.0982 mL4.1963 mL20.9815 mL
10 mM0.2098 mL1.0491 mL2.0982 mL10.4908 mL
20 mM0.1049 mL0.5245 mL1.0491 mL5.2454 mL
50 mM0.0420 mL0.2098 mL0.4196 mL2.0982 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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