fluorescein biotin

Fluorescein Biotin is a biotin-substituting fluorescent dye that detects and quantifies biotin binding sites by fluorescence or absorbance.Fluorescein Biotin undergoes fluorescence quenching when bound to avidin or streptavidin.

Biotin (5-fluorescein) conjugate (N-(Fluorescein-5-carbamidoethyl)biotinamide) is a fluorescent biotin derivative known for its high efficiency in cell labeling. This conjugate is utilized in biomedical research to detect and localize target molecules. It is extensively used in immunofluorescence staining experiments to enhance sensitivity and specificity.

Flubi-2 is a fluorescein-biotin-based pH ratio dye (Ex=480 nm, Em=520-560 nm) with a pKa of 6.7. It is the hydrolysis product of Flubida-2 (non-fluorescent with membrane permeability). Flubi-2 is applicable for measuring pH in organelles of the secretory pathway in live cells, such as the Golgi apparatus and endoplasmic reticulum.

Biotin-4-Fluorescein is a high-affinity fluorescent tracer probe consisting of biotin covalently linked to a fluorescein molecule. Leveraging the exceptionally strong non-covalent interaction between biotin and avidin or streptavidin, this compound is widely used in competitive binding assays for the quantitative analysis of the binding affinities of biotin and its derivatives. The significant quenching of its fluorescence upon binding to avidin makes it an ideal molecular tool for kinetic studies and high-throughput screening.