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Flubi-2

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Catalog No. T208385

Flubi-2 is a fluorescein-biotin-based pH ratio dye (Ex=480 nm, Em=520-560 nm) with a pKa of 6.7. It is the hydrolysis product of Flubida-2 (non-fluorescent with membrane permeability). Flubi-2 is applicable for measuring pH in organelles of the secretory pathway in live cells, such as the Golgi apparatus and endoplasmic reticulum.

Flubi-2

Flubi-2

😃Good
Catalog No. T208385
Flubi-2 is a fluorescein-biotin-based pH ratio dye (Ex=480 nm, Em=520-560 nm) with a pKa of 6.7. It is the hydrolysis product of Flubida-2 (non-fluorescent with membrane permeability). Flubi-2 is applicable for measuring pH in organelles of the secretory pathway in live cells, such as the Golgi apparatus and endoplasmic reticulum.
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Flubi-2 is a fluorescein-biotin-based pH ratio dye (Ex=480 nm, Em=520-560 nm) with a pKa of 6.7. It is the hydrolysis product of Flubida-2 (non-fluorescent with membrane permeability). Flubi-2 is applicable for measuring pH in organelles of the secretory pathway in live cells, such as the Golgi apparatus and endoplasmic reticulum.
Chemical Properties
FormulaC78H88N8O16S2
Storage & Solubility Information
Storagekeep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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