2-acetamidophenol

2-Acetamidophenol (Orthocetamol) has analgesic and antipyretic effects. 2-Acetamidophenol is an isomer of Paracetamol (4-acetamidophenol).

2-Acetamidophenol-d3 (Orthocetamol-d3) is the deuterated form of 2-Acetamidophenol. This compound (Orthocetamol) acts as a regulator targeting both ferroptosis and the glutathione metabolism pathway and serves as the ortho-isomer of Paracetamol. It exhibits anti-atherosclerotic activity, with IC50 values of 30 μM and 40 μM for inhibiting total cholesterol (TC) and triglycerides (TG) in a zebrafish hyperlipidemia model, respectively. 2-Acetamidophenol enhances glutathione peroxidase GPX4 activity, lowers intracellular reactive oxygen species (ROS) and ferrous ion (Fe2+) accumulation, and upregulates the expression of genes related to glutathione synthesis (such as GCLC, GCLM, GSS) and iron ion transport (including FPN1, FTH), thereby inhibiting ox-LDL uptake by macrophages and foam cell formation.

Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.