znaf-1

ZnAF-1, a zinc sensor compound, utilizes a fluorescein-based structure incorporating the N,N-bis(2-pyridylmethyl)ethylenediamine chelating unit. Its primary function lies in the detection of Zn2+ ions. Through its binding capability, ZnAF-1 achieves a 1:1 stoichiometry with Zn(II).

ZnAF-1F, with a Kd value of 2.2 nM, serves as a powerful fluorophore and is effective as fluorescent probes for Zn2+ in cellular applications. It exhibits an excitation wavelength (λ excitation) of 489 nm and an emission wavelength (λ emission) of 514 nm [1] [2] [3].

ZnAF-2, 6-iso- is a cell-impermeable, high-affinity Zn2-specific fluorescent probe (Kd = 2.7 nM) that remains relatively non-fluorescent until it binds to Zn2 in a stoichiometric (1:1) ratio, resulting in an approximately 51-fold increase in fluorescence intensity. It shows minimal affinity for Ca2, Mg2, Na, or K.