reticulocytes

Thiazole Orange is a dye that can be used for reticulocyte analysis.

(Z)-Thiazole Orange iodide is an asymmetric cyanine dye with fluorescence highly dependent on its local environment. In solution, it is generally non-fluorescent; however, its fluorescence increases by a factor of one thousand when bound to double-stranded DNA and RNA (dsDNA or dsRNA). The compound's absorption maxima while complexed with DNA is 509 nm, and its emission maxima is 532 nm. (Z)-Thiazole Orange iodide solutions are widely utilized for determining the percentage of reticulocytes in human peripheral blood using microscopy and flow cytometry.

8-Azidoadenosine-5'-O-diphosphate (8-azido-ADP), a clickable variant of the ATP precursor and purinergic receptor ligand ADP, serves as a photoaffinity label for proteins including glutamate dehydrogenase (GDH) isoproteins from bovine brains, as well as eukaryotic translation initiation factor 2 (eIF2) and guanine nucleotide exchange factor (GEF) from rabbit reticulocytes. Radiolabeled versions of this compound facilitate the identification of these protein interactions.