raw 264.7 macrophage

E17241 functions as an inducer of ABCA1 gene expression, effectively increasing its expression with an EC50 value of 280 nM. It also acts as an agonist for peroxisome proliferator-activated receptors (PPARs), inducing PPAR-mediated gene expression in HepG2 cells expressing PPARγ, PPARα, or PPARδ, with respective EC50 values of 290, 3,900, and 879 nM. In RAW 264.7 macrophage cells, E17241 enhances ACBA1 protein levels, although this effect is absent when siRNA targeting PKCζ mRNA is present. Treatment with E17241 (0.4, 2, or 10 µM) increases cholesterol efflux in RAW 264.7 cells. In an atherosclerosis model using ApoE-/- mice administered with a dose of 25 mg/kg, E17241 reduces levels of plasma cholesterol, alanine transaminase (ALT), aspartate transaminase (AST), and liver cholesterol and triglycerides, and also decreases the area of aortic lesions. Additionally, daily administration of E17241 (50 mg/kg) lowers blood glucose levels and body weight in KKAy diabetic mice on a high-fat, high-glucose (HFHG) diet.

EP4 receptor antagonist 7 (Compound 14) is an antagonist of the prostaglandin E2 (PGE2) receptor subtype EP4, with an IC50 of 1.1 nM. This compound inhibits PGE2-induced β-arrestin recruitment in HEK293 cells with an IC50 of 0.9 nM. In RAW 264.7 macrophages, it reduces the expression of PGE2-induced IL-4, macrophage mannose receptor 1 (Mrc1), chitinase-like protein 3 (Chil3), chemokine (C-X-C motif) ligand 1 (Cxcl1), triggering receptor expressed on myeloid cells 2 (Trem2), and arginase 1 (Arg1) mRNA. In the CT26 mouse colon cancer model, EP4 receptor antagonist 7, combined with an anti-PD-1 antibody, inhibits tumor growth and enhances CD8+ T cell infiltration into the tumor.

Madecassic acid (L-fucopyranose) is extracted from the whole herb of Centella asiatica.

Cimifugin (Cimitin) inhibits NO production induced by lipopolysaccharide in macrophage cell line RAW 264.7 and for 1, 1-diphenyl-2-picrylhydrazyl free-radical scavenging activity in cell-free bioassay system.

GGTI-2166 is a geranylgeranyl transferase I inhibitor. GGTI-2166 inhibit the pOC formation induced by RANKL or TNF-alpha in cultures of both mouse marrow-derived macrophage-colony-stimulating factor (M-CSF) dependent monocytes (MD cells) and the mouse monocyte cell line RAW 264.7 (RAW cells). GGTI-2166 inhibited TRAP activity induced by RANKL or TNF-alpha in both cell cultures and prevented the incorporation of [3H]all-trans geranylgeraniol into prenylated proteins in RAW cells.

Hyperectumine demonstrates moderate anti-inflammatory efficacy by inhibiting the activity of inflammatory mediators induced by LPS in RAW 264.7 macrophage cells.

EP4 antagonist 14 is a prostaglandin E2 receptor subtype EP4 antagonist, demonstrating an IC50 of 1.1 nM in a HEK293 human receptor reporter assay and blocking β-arrestin recruitment due to PGE2 with an IC50 of 0.9 nM. At 10 µM, it reduces PGE2-stimulated mRNA levels of Il-4, macrophage mannose receptor 1 (Mrc1), chitinase-like protein 3 (Chil3), chemokine (C-X-C) motif ligand 1 (Cxcl1), triggering receptor expressed on myeloid cells 2 (Trem2), and arginase-1 (Arg1) in RAW 264.7 macrophages. When used at 30 mg/kg per day alongside anti-PD-1 antibodies, it curbs tumor growth and enhances CD8+ T cell infiltration in tumors using a CT26 murine colon cancer model.

3,4-Dihydro-6,7-isoquinolinediol (compound 7) acts as an agonist for β2-AR, demonstrating an EC50 of 106.9 μM in CH-K1/GA1S cells. Additionally, this compound exhibits anti-inflammatory activity by inhibiting the production of nitric oxide (NO) in lipopolysaccharide-induced RAW 264.7 murine macrophage cells.

Cimifugin (Standard) is a reference standard for research and analysis in studies involving Cimifugin. Cimifugin (Cimitin) inhibits NO production induced by lipopolysaccharide in macrophage cell line RAW 264.7 and for 1, 1-diphenyl-2-picrylhydrazyl free-radical scavenging activity in cell-free bioassay system.

Harzianoside B is a diterpene aminoglycoside compound identified from Trichoderma sp. SCSIOW21. This compound shows activity in inhibiting NO production, with an IC50 value near the maximum tested concentration of 100 µM in RAW 264.7 macrophage cell assays. It is applicable in anti-inflammatory research.

Cycloneroside E is a sesquiterpene aminoglycoside compound isolated from Trichoderma sp. SCSIOW21. It exhibits activity in inhibiting NO production, with an IC50 value of 48.0 µM in macrophage RAW 264.7 cells. Cycloneroside E can be used in anti-inflammatory research.

Trichaspside F, a sesquiterpene aminoglycoside compound, was isolated from Trichoderma sp. SCSIOW21. It exhibits activity in inhibiting NO production, with an IC50 value of 54.8 µM in RAW 264.7 macrophage cells. Trichaspside F holds potential for research in the anti-inflammatory field.

Cycloneroside A is a sesquiterpene aminoglycoside compound identified from Trichoderma sp. SCSIOW21. It exhibits activity in inhibiting nitric oxide (NO) production, with an IC50 value of approximately 100 µM in RAW 264.7 macrophage cells. Cycloneroside A is applicable in anti-inflammatory research.

Cycloneroside C is a novel sesquiterpene aminoglycoside compound discovered in the deep-sea sediment fungus Trichoderma sp. SCSIOW21. This compound exhibits activity in inhibiting NO production, with an IC50 value of 57.1 μM in macrophage RAW 264.7 cell assays. Cycloneroside C can be utilized for research in the field of anti-inflammatory studies.

Cycloneroside D, a novel sesquiterpene aminoglycoside, was isolated from the deep-sea sediment fungus Trichoderma sp. SCSIOW21. It demonstrates activity in inhibiting NO production, with an IC50 value of 42.0 μM in macrophage RAW 264.7 cell experiments. Cycloneroside D is a potential candidate for anti-inflammatory research.

7,3',4'-Tri-O-methylluteolin (5-Hydroxy-3',4',7-trimethoxyflavone) exhibits anti-inflammatory, antibacterial, and antifungal activities, along with moderate antitrypanosomal activity, having an MIC value of 19.0 µg/mL.

Dactylorhin A, a succinate derivative ester isolated from the rhizomes of Gymnadenia conopsea, exhibits a moderate inhibitory effect on nitric oxide (NO) production in RAW 264.7 macrophage cells.

Rutaevin shows the inhibitory activity on nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 macrophages, it may be as a valuable anti-inflammatory agent.

Futokadsurin C can inhibit nitric oxide production by a murine macrophage-like cell line (RAW 264.7), which is activated by lipopolysaccharide and interferon-gamma.