methyl 3,4,5trimethoxycinnamate

Methyl 3,4,5-trimethoxycinnamate (MTC) is a bioactive natural phenylpropanoid. Methyl 3,4,5-trimethoxycinnamate (MTC) was evaluated in RAW264.7 macrophages and RAW264.7-3T3-L1 adipocyte co-culture systems using ELISAs, Griess assay, enzyme immunoassays, cytoblot analysis, DNA binding assays, reporter gene assays, and glucose uptake assays to investigate inflammatory and metabolic signaling responses. Methyl 3,4,5-trimethoxycinnamate (MTC) at 5–20 µM suppressed LPS + IFNγ-induced release of TNFα, IL-6 and IL-1β, as well as NO/iNOS and PGE2/COX-2 levels in RAW264.7 cells. Methyl 3,4,5-trimethoxycinnamate (MTC) also reduced phospho-IκB and phospho-p65 protein levels and total IκB levels in RAW264.7 cells, and is therefore used in immunometabolic signaling research to model inflammatory transcriptional regulation in macrophage–adipocyte co-culture systems.