fluorophore

7-Amino-4-methylcoumarin-3-acetic acid [7-Amino-4-methylcoumarin] is a more suitable substrate for enteropeptidase compared to GD(4)K-NA.

GLF16 HCl is a fluorophore-coupled Sudan Black B analog that allows rapid detection, isolation and real-time tracking of senescent cells by fluorescence microscopy and flow cytometry.

YK-5-252 is a dual action combretastatin A-4 (CA-4) prodrug which releases CA-4 through a disulfide bond cleavage mechanism and contains a near-infrared (NIR) fluorophore.

The renin fluorogenic substrate consists of the normal peptide substrate for renin which has been linked to the fluorophore EDANS at one end and to a non-fluorescent quenching molecule (Dabcyl) at the other. After cleavage by renin, the product (peptide-EDANS) is brightly fluorescent and can be easily analyzed using an excitation wavelength of 340 nm and emission wavelengths of 485-510 nm.

N-Descyclopropanecarbaldehyde Olaparib, an Olaparib analogue, incorporates a DOTA moiety. It acts as a CRBN-based ligand for the formation of novel dual EGFR and PARP PROTAC, DP-C-4[1]. N-Descyclopropanecarbaldehyde Olaparib is suitable for radiolabeling with F-18 or a fluorophore to visualize tumors using positron emission tomography (PET) or optical imaging[2].

CAY10732 is a fluorophore for the detection of hydrogen sulfide for H2S imaging in living cells.

Coumarin-quinone conjugate is a fluorescent substrate for NADH:ubiquinone oxidoreductases.1It is comprised of a coumarin fluorophore conjugated to a ubiquinone analog that can be reduced by NADH:ubiquinone oxidoreductases. It has been used to measure the kinetic parameters of apoptosis-inducing factor mitochondria-associated 2/ferroptosis suppressor protein 1 (AIFM2/FSP1). 1.Doll, S., Freitas, F.P., Shah, R., et al.FSP1 is a glutathione-independent ferroptosis suppressorNature575(7784)693-698(2019)

TFMU-ADPr triethylamine, a versatile substrate for evaluating poly(ADP-ribose) glycohydrolase (PARG) activity, enables direct measurement of total PAR hydrolase activity through the liberation of a fluorophore. With outstanding reactivity, generality, stability, and usability, it serves as an essential tool for the in vitro assessment of small-molecule inhibitors and exploration of the regulation of ADP-ribosyl catabolic enzymes [1].

Vem-L-Cy5 (compound 3), a Vemurafenib-based BRAF inhibitor conjugated with the near-infrared (NIR) fluorophore cyanine-5 (Cy5), selectively targets the BRAF V600E mutation and inhibits MEK phosphorylation. This compound demonstrates cell permeability and has been shown to inhibit the growth of various cancer cell types [1].

TNO211 is a bioactive peptide that functions as a highly soluble fluorogenic substrate for select Matrix Metalloproteinases (MMPs)—specifically MMP-2, 8, 12, 13, and 14. Incorporating a cleavable Gly-Leu bond and an EDANS DABCYL fluorophore quencher pair, TNO211 enables sensitive detection of MMP activity through fluorescence assays, with an absorption emission profile at 340 490 nm. It is particularly effective in analyzing MMPs in culture media from endothelial cells as well as untreated synovial fluid from patients, highlighting its utility in investigations of MMP-associated cellular behaviors, including proliferation, migration (adhesion dispersion), differentiation, angiogenesis, apoptosis, and host defense.

Diacylglycerols (DAG) are generated through the hydrolysis of membrane phospholipids and function as lipid second messengers by activating protein kinase C (PKC) and modulating cell growth and apoptosis. Additionally, they act as precursors for DAG kinases in the synthesis of phosphatidic acid, a crucial lipid messenger. The compound 1-NBD-decanoyl-2-decanoyl-sn-glycerol incorporates a nitrobenzoxadiazole (NBD) fluorophore at the ω-end of its terminal decanoyl chain, using the structure of 1,2-didecanoyl-sn-glycerol as a model for diacylglycerol. This molecule is expected to exhibit excitation and emission peaks at roughly 470/541 nm, paralleling those of various NBD-labeled phospholipids. Fluorescently labeled lipids, such as this, are instrumental in exploring their interactions with proteins, their uptake by cells and liposomes, and in developing assays for lipid metabolism research.

Cy5.5 DBCO, a click chemistry reagent, features a cycloalkyne group and serves as a linker for the Cyanine5.5 fluorophore. Its DBCO group facilitates copper-free, biocompatible click chemistry, characterized by rapid reaction kinetics and high stability [1].

DOPE-PEG-BDP FL, MW 5000 is a PEG-lipid-dye conjugate composed of an unsaturated phospholipid, DOPE, a BDP FL fluorophore with excitation and emission maxima at 504 nm and 514 nm, respectively, and a large PEG spacer that links the phospholipid and fluorophore.

DSPE-PEG-Cy3, MW 2000 is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits peak absorption at wavelengths between 548-552 nm and maximum emission between 562-570 nm.

DSPE-PEG-Cy3, MW 3400, is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is frequently utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits a peak absorption wavelength in the range of 548-552 nm and a peak emission wavelength between 562-570 nm.

DSPE-PEG-Cy3, MW 5000, is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits peak absorption at wavelengths between 548-552 nm and peak emission between 562-570 nm.

DSPE-PEG-Cy5, MW 2000, is a PEG lipid conjugated with a fluorescent group. The Cy5 fluorophore is commonly applied in the labeling of proteins and nucleic acids, suitable for imaging, flow cytometry, and genomic applications. The dye features an excitation peak at 651 nm and an emission peak at 670 nm.

DSPE-PEG-Cy5, MW3400, is a PEG lipid conjugated with a fluorescent group. The Cy5 fluorophore is commonly utilized for labeling proteins and nucleic acids, facilitating imaging, flow cytometry, and genomic applications. The dye exhibits an excitation peak at 651 nm and an emission peak at 670 nm.

DSPE-PEG-Cy5, MW5000, is a PEG lipid conjugated with a fluorescent group. The Cy5 fluorophore is commonly utilized for protein and nucleic acid labeling for imaging, flow cytometry, and genomic applications. The dye exhibits an excitation peak at 651 nm and an emission peak at 670 nm.

(Z)-Non-2-enyl 6-bromohexanoate is an analogue of biotin and acts as a protein cross-linker. It exhibits weak binding to biotin-binding proteins (such as avidin) and can be easily displaced by biotin. This compound is used in the preparation of agarose matrices for the affinity-based separation of streptavidin-fluorophore conjugates.

Bovine Serum Albumin-PE is a biochemical reagent conjugated with the fluorophore PE (Ex is 565 nm; Em is 578 nm). This compound is utilized for labeling or detecting specific antigens, molecules, or cell structures.

Human Serum Albumin-AF488 is a biochemical reagent conjugated with the fluorophore AF488 (Ex=488 nm; Em=496 nm). It is utilized for labeling or detecting specific antigens, molecules, or cellular structures.