BI 2852 FL is a cell-permeable bioluminescence resonance energy transfer (BRET) fluorescent probe targeting the pan-RAS type. It is synthesized by conjugating a fluorophore with a derivative of the reversible SI/II-P KRAS inhibitor, BI 2852. BI 2852 FL is employed in a competitive BRET format to quantify the binding of SII-P to multimeric RAS complexes within living cells.

BI 2852 FL functions as a cell-permeable pan-RAS bioluminescence resonance energy transfer (BRET) fluorescent probe. It is crafted through the conjugation of a fluorophore to a derivative of BI 2852, the reversible SI/II-P KRAS inhibitor. Employed in a competitive BRET format, BI 2852 FL is utilized to assess SII-P engagement with multimeric RAS complexes within live cells.