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AF555 NHS ester (Synonyms: Alexa Fluor 555 NHS ester)
Catalog No. T212863 Copy Product Info
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AF555 NHS Ester is a bright orange-red fluorescent probe containing the AF555 fluorophore and an NHS active ester group. It efficiently forms covalent bonds with primary amines (R‑NH₂) in biomolecules, enabling stable labeling and tracking of target molecules. This reagent is suitable for fluorescent labeling of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Its excitation/emission maxima are 555/572 nm, making it widely used in bioimaging, immunoassays, and other life science applications.
AF555 NHS ester
Copy Product Info😃Good
Catalog No. T212863
Synonyms Alexa Fluor 555 NHS ester
AF555 NHS Ester is a bright orange-red fluorescent probe containing the AF555 fluorophore and an NHS active ester group. It efficiently forms covalent bonds with primary amines (R‑NH₂) in biomolecules, enabling stable labeling and tracking of target molecules. This reagent is suitable for fluorescent labeling of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Its excitation/emission maxima are 555/572 nm, making it widely used in bioimaging, immunoassays, and other life science applications.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | AF555 NHS Ester is a bright orange-red fluorescent probe containing the AF555 fluorophore and an NHS active ester group. It efficiently forms covalent bonds with primary amines (R‑NH₂) in biomolecules, enabling stable labeling and tracking of target molecules. This reagent is suitable for fluorescent labeling of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Its excitation/emission maxima are 555/572 nm, making it widely used in bioimaging, immunoassays, and other life science applications. |
| Synonyms | Alexa Fluor 555 NHS ester |
| Molecular Weight | 1058.35 |
| Formula | C39H46K3N3O16S4 |
| Smiles | O=S(C1=CC2=C([N+](CCCS(=O)([O-])=O)=C(/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C5=C(C=C(S(=O)(O[K])=O)C=C5)C/3(C)C)C2(CCCCS(=O)(O[K])=O)C)C=C1)(O[K])=O |
| Storage | Keep away from direct sunlight | Store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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