dpnb-abt594

DPNB-ABT594, a nitrobenzyl-caged ABT594, selectively activates nAChRs containing α4β2 subunits over the α7 subunit. It is utilized to map the distribution of nAChRs on medial habenula (MHb) neurons, thereby facilitating a deeper understanding of nAChR-mediated Ca2+ signaling in the MHb [1].

DPNB-ABT 594 is a nitrobenzyl-caged ABT 594, a selective α4β2 nAChR agonist. One-photon uncaging evokes large inward currents and Ca2+transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices. Two-photon uncaging induces fast nAChR-mediated currents. Photolyzed with high quantum yield of 0.20. Effective photolysis occurs using one- or two-photon excitation; one-photon uncaging requires illumination at 410 nm for 1.5-3 ms; two-photon uncaging requires illumination with appropriate two photon pulse laser at 710 nm for ~2 ms.