8 azido atp

8-Azido-ATP is a nucleotide analog that demonstrates photo-reactivity properties. It offers utility in the precise labeling and identification of proteins, particularly those involved in DNA-dependent RNA polymerase activity.

8-Azido-ATP (8-N3-ATP) trisodium is a photolabile nucleotide analog used for identifying proteins such as DNA-dependent RNA polymerase. As a click chemistry reagent, 8-Azido-ATP trisodium features an azide group that can engage in copper-catalyzed azide-alkyne cycloaddition reactions (CuAAc) with alkyne-containing molecules. Additionally, it can participate in strain-promoted azide-alkyne cycloaddition reactions (SPAAC) with molecules containing DBCO or BCN groups.

8-Azidoadenosine-5'-O-diphosphate (8-azido-ADP), a clickable variant of the ATP precursor and purinergic receptor ligand ADP, serves as a photoaffinity label for proteins including glutamate dehydrogenase (GDH) isoproteins from bovine brains, as well as eukaryotic translation initiation factor 2 (eIF2) and guanine nucleotide exchange factor (GEF) from rabbit reticulocytes. Radiolabeled versions of this compound facilitate the identification of these protein interactions.