8-Azido-ATP

Name: 8-Azido-ATP
Brand: TargetMol
SKU: T40616
Rating: 4.5 (1 reviews)

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Catalog No. T40616Cas No. 53696-59-6

Alias 8-N3-ATP, 8-Azido-ATP, 8-Azidoadenosine 5'-triphosphate

8-Azido-ATP is a nucleotide analog that demonstrates photo-reactivity properties. It offers utility in the precise labeling and identification of proteins, particularly those involved in DNA-dependent RNA polymerase activity.

8-Azido-ATP

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Catalog No. T40616Alias 8-N3-ATP, 8-Azido-ATP, 8-Azidoadenosine 5'-triphosphateCas No. 53696-59-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	Inquiry	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	8-Azido-ATP is a nucleotide analog that demonstrates photo-reactivity properties. It offers utility in the precise labeling and identification of proteins, particularly those involved in DNA-dependent RNA polymerase activity.
In vitro	8-Azido-ATP (azido-ATP) serves as an effective tool in identifying viral RNA polymerase by inhibiting transcriptional activity in rotavirus particles upon UV exposure. This compound reduces viral transcription in a dose-dependent fashion. Additionally, 8-Azido-ATP exhibits a reduced potency in blocking Kir6.2ΔC26 currents compared to ATP, with a half-maximal inhibition (Ki) observed at 2.8 ± 0.4 mM for 8-azido-ATP versus 172 ± 7 mM for ATP, indicating a lower efficacy. The Hill coefficients for 8-azido-ATP and ATP are 0.9 ± 0.2 and 1.3 ± 0.1, respectively, suggesting differences in binding affinity.
Synonyms	8-N3-ATP, 8-Azido-ATP, 8-Azidoadenosine 5'-triphosphate

Chemical Properties

Molecular Weight	548.19
Formula	C₁₀H₁₅N₈O₁₃P₃
Cas No.	53696-59-6
Smiles	Nc1ncnc2n([C@@H]3O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]3O)c(N=[N+]=[N-])nc12

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc