6aminofluorescein

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

Ac4ManNDAz is a cell-permeable photocrosslinking probe that is a precursor to photocrosslinked sialic acid, which attaches to cell surface α2-6-conjugated sialic acid and produces photocrosslinking via sialic acid.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

Alizarin Cyanine Green F (NSC-41838) is an agent of green dye. It can be used to dye paper, chromed leather, wool, silk, and nylon (acid bath), to stain wood, and to color anodized aluminum, urea-melamine resin, and soap. It is used to color nylon 66 and or nylon 6 nonabsorbable surgical sutures for use in general surgery and used for coloring drugs generally, including drugs intended for use in the area of the eye. Alizarin Cyanine Green F is also proposed for use in semi-permanent hair dye formulations as a direct dye at a maximum concentration of 0.3% in the finished cosmetic product.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Laurdan (6-Dodecanoyl-2-dimethylaminonaphthalene) is a fluorescent membrane probe that is spectrally sensitive to the phospholipid phase of the cell membrane to which it binds.

AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate) is a reagent used in HPLC for the fluorescence detection of amino acid or protein sequences. It reacts with primary and secondary amino acids to produce fluorescent derivatives, enabling the detection of amino acids at picomolar levels.

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein. 6-FAM SE is mainly used for labeling nucleotides and sequencing nucleic acids.

DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid) is an amine-reactive building block recognized by anti-DNP antibodies and serves as an excellent amine-reactive FRET quencher when paired with Trp or Tyr.

6-ROX (6-Carboxy-X-rhodamine) is a fluorescent oligonucleotide marker and acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging with excitation of 514nm and emission of 525nm.

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

AMCA-6-dUTP is suitable for the enzymatic labeling of DNA cDNA [1].

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a quenched, fluorescent MMP peptide substrate designed for real-time quantification of MMP enzymatic activity. As an extended variant of the MMP substrate (FS-1), it demonstrates increased specificity against collagenases (MMP-1, MMP-8, MMP-13) and MT1-MMP, with higher specificity constants (Ex Em=325 nm 400 nm). [1]

6-TET Azide serves as a fluorescent probe, useful in developing fluorescent contrast agents and for nucleic acid sequencing [1].

Ac-ANW-AMC is a fluorogenic substrate for the β5i LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released, and its fluorescence quantifies the activity of the β5i LMP7 subunit. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively.

Direct Green 6 is a green dye.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

6-TET phosphoramidite (5′-Tetrachlorofluorescein phosphoramidite) serves as a fluorescent dye to label oligonucleotides with fluorescein [1].

6-HEX (SE) is a derivative of 6-HEX widely used in nucleic acid sequencing [1].

6-Iodoacetamidofluorescein is a sulphhydryl-specific fluorescent dye utilized for selective labeling of -SH groups present in nuclear matrix polypeptides and proteins.

ROX NHS ester, 6-isomer, is a highly fluorescent and photostable rhodamine dye used for labeling amino groups in peptides, proteins, and amino-oligonucleotides. ROX-labeled oligonucleotide probes are commonly used in qPCR, and qPCR instruments have a ROX channel. [Pure single isomer.]