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6-Aminofluorescein

Name: 6-Aminofluorescein
Brand: TargetMol
SKU: T18900
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18900Cas No. 51649-83-3

Alias 6-AF

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

6-Aminofluorescein

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Purity: 99.26%

Catalog No. T18900Alias 6-AFCas No. 51649-83-3

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$43	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.26%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.
In vitro	Instructions 1. Preparation of reaction system: 1) 6-AF solution: Prepare 6-AF stock solution using appropriate solvent (e.g. DMSO or PBS), usually at a concentration of 1–10 mM. 2) Prepare target molecules (e.g. enzymes or metabolites) in buffer. 3) Add 6-AF as a fluorescent probe, usually at a concentration of 1–100 µM. 2. Detection process: Record fluorescence signal using a fluorometer or microplate reader (excitation wavelength 495 nm, emission wavelength 515 nm). 3. Data analysis: Compare the fluorescence intensity of the experimental group and the control group to evaluate the dynamic changes of the target molecule or reaction. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	6-AF

Chemical Properties

Molecular Weight	347.32
Formula	C₂₀H₁₃NO₅
Cas No.	51649-83-3
Smiles	Nc1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31
Relative Density.	1.64 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (259.13 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (9.5 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8792 mL	14.3959 mL	28.7919 mL	143.9595 mL
5 mM	0.5758 mL	2.8792 mL	5.7584 mL	28.7919 mL
10 mM	0.2879 mL	1.4396 mL	2.8792 mL	14.3959 mL
20 mM	0.1440 mL	0.7198 mL	1.4396 mL	7.1980 mL
50 mM	0.0576 mL	0.2879 mL	0.5758 mL	2.8792 mL
100 mM	0.0288 mL	0.1440 mL	0.2879 mL	1.4396 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Inhibitorinhibit6-Aminofluorescein6Aminofluorescein6 Aminofluorescein