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6-Aminofluorescein

Name: 6-Aminofluorescein
Brand: TargetMol
SKU: T18900
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18900Cas No. 51649-83-3

Alias 6-AF

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

6-Aminofluorescein

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Purity: 99.26%

Catalog No. T18900Alias 6-AFCas No. 51649-83-3

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

Pack Size	Price	Availability	Quantity
100 mg	$43	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock

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Purity:99.26%

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COA HNMR HPLC

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.
In vitro	Instructions 1. Preparation of reaction system: 1) 6-AF solution: Prepare 6-AF stock solution using appropriate solvent (e.g. DMSO or PBS), usually at a concentration of 1–10 mM. 2) Prepare target molecules (e.g. enzymes or metabolites) in buffer. 3) Add 6-AF as a fluorescent probe, usually at a concentration of 1–100 µM. 2. Detection process: Record fluorescence signal using a fluorometer or microplate reader (excitation wavelength 495 nm, emission wavelength 515 nm). 3. Data analysis: Compare the fluorescence intensity of the experimental group and the control group to evaluate the dynamic changes of the target molecule or reaction.
Alias	6-AF

Chemical Properties

Molecular Weight	347.32
Formula	C₂₀H₁₃NO₅
Cas No.	51649-83-3
Smiles	Nc1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31
Relative Density.	1.64 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 90 mg/mL (259.13 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8792 mL	14.3959 mL	28.7919 mL	143.9595 mL
5 mM	0.5758 mL	2.8792 mL	5.7584 mL	28.7919 mL
10 mM	0.2879 mL	1.4396 mL	2.8792 mL	14.3959 mL
20 mM	0.1440 mL	0.7198 mL	1.4396 mL	7.1980 mL
50 mM	0.0576 mL	0.2879 mL	0.5758 mL	2.8792 mL
100 mM	0.0288 mL	0.1440 mL	0.2879 mL	1.4396 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Inhibitorinhibit6-Aminofluorescein6Aminofluorescein6 Aminofluorescein