Malachite green (Solid Green Crystals O) is an N-methylated diaminotriphenylmethane dye used for coloring.

Blue Tetrazolium (Tetrazolium blue), a dye used in microbial research, can be reduced to blue tetrazolium formazan (BTF). It determines succinate dehydrogenase (SDH) activity in yeast strains as a reduced substrate. Extract BTF using DMSO and test its absorption spectrum. BTF exhibits a wavelength range of 480-600 nm with maximal absorbance at 540 nm [1].

Cellufluor is a fluorescent whitening agent widely used in the textile and paper industries, as well as in biological research. It specifically binds to polysaccharides containing β-1,3 and β-1,4 glycosidic bonds (such as cellulose and chitin), producing strong fluorescence when excited by ultraviolet or short-wavelength visible light, and is used to visualize cell wall structures. In microbiology, Cellufluor enables rapid and sensitive detection of chitin and cellulose in the cell walls of fungi, algae, and plants. Due to the absence of cell wall polysaccharide structures, Cellufluor typically does not stain mammalian cells.