rhodff (potassium salt)

Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 μM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively.

Rhod-FF tripotassium is a derivative of Rhod-2 and serves as a low-affinity calcium ion indicator (Kd = 320 μM). It signals calcium ion concentration by binding with calcium ions and undergoing fluorescence changes, with excitation at 552 nm and emission at 580 nm.