fluo-4,

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-4 AM is a cell-permeable calcium ion (Ca²⁺) fluorescent indicator/chemical probe suitable for fluorescence imaging, flow cytometry, and plate screening, with an excitation wavelength of approximately 488 nm and an emission wavelength of 512–520 nm.

Fluo-4 potassium is a cell-permeant Ca2+ probe (λex=494 nm, λem=516 nm).

Fluo-4FF AM is a cell-permeable fluorescent calcium indicator. As an analog of Fluo-4, Fluo-4FF has lower affinity for calcium, making it suitable for investigating conditions with elevated intracellular calcium levels.

Mag-Fluo-4 AM is a Mg 2+ (magnesium ion) dye and a low-affinity Ca 2+ (calcium ion) dye and fluorescent indicator with Kd of 4.7 mM and 22 µM, respectively, that localizes Ca 2+ in the lumen of endoplasmic reticulum-like tubules in many cell types, and is commonly used in live cell assays.