d luciferin sodium

D-Luciferin Sodium is a specific natural substrate for firefly luciferase that catalyzes the production of characteristic yellow-green light in bioluminescent insects. It serves as a core tool for in vivo imaging, cell-level reporter gene detection, and analysis of luciferase activity in tissues and living organisms. The luciferase (luc) gene is a commonly used reporter gene for research and active molecule screening. Chemiluminescence technology is essentially background-free, making the luc reporter gene an ideal choice for detecting low levels of gene expression. Luciferase activity as low as 0.02 pg can be reliably measured in a standard fluorometer.

D-Luciferin 6'-methyl ether sodium salt functions as an inhibitor of firefly luciferase and acts as a cofactor in the Luc-catalyzed synthesis of dinucleoside polyphosphates [1] [2].