copper probe cf4

Copper probe CF4 (Copper fluor CF4) is a monovalent copper (Cu+) specific fluorescent sensor based on a rhodol dye scaffold, featuring high copper selectivity with a Kd value of 2.9×10−13 M. Copper Probe CF4 has a probe-to-substrate stoichiometric ratio of 1:1 and is stable within a pH range of 6–8. Copper Probe CF4 can be used for copper imaging and copper level measurement in small organisms; Ex/Em = 415/660 nm.

Ctrl-CF4-S2 is a control probe for the chemically modified Copper Fluor-4 (CF4), in which two of the four thioether ligands of the CF4 probe are replaced with methylene groups. Unlike CF4, which is a fluorescent probe used to detect the presence and distribution of copper ions, Ctrl-CF4-S2 does not respond to copper ions. This lack of response helps eliminate background signals from copper ions, thereby confirming whether the signals from CF4 accurately reflect the dynamic changes of copper ions within biological systems.