c3h10t1/2 cells

MRT-81 is a potent antagonist of human and rodent smoothened receptors that inhibits hedgehog activity with an IC50 value of 41 nM in Shh-light2 cells.It is used in the study of cancer.

Picoberin is a potent inhibitor at low picomolar concentrations, effectively blocking osteoblast differentiation induced by the Hedgehog signaling pathway. As a strong aryl hydrocarbon receptor (AhR) agonist, it also inhibits Wnt3A-induced osteoblast differentiation in C3H10T1/2 cells. Additionally, Picoberin upregulates genes encoding phase I and II metabolic enzymes.

HSP70-IN-3, a potent inhibitor of HSP70 with IC50 values of 1.1 μM in ASZ001 cells and 1.9 μM in C3H10T1/2 cells, exhibits anti-Hedgehog (Hh) signaling and anti-proliferative activities. Furthermore, it reduces the expression of the oncogenic transcription factor GLI1 [1].

Hedgehog IN-2 (Compound 20) acts as an inhibitor of the Hedgehog signaling pathway, with an IC50 of <0.003 μM in C3H10T1/2 cells [1].