9(r)hete

9(R)-HETE, which constitutes 50% of (±)9-HETE, activates RXRγ-dependent transcription 1.5-fold relative to a control at a concentration of 300 nM. The stereochemical assignment of the (R) enantiomer is based on the comparison of chiral HPLC retention times to published results.

(±)9-HETE is one of the six monohydroxy fatty acids produced by the non-enzymatic oxidation of arachidonic acid. The individual R and S isomers of racemic HETEs have been separated and identified using chiral phase HPLC. The racemic HETEs have been quantified as an index of lipid peroxidation using GC/MS.