7 methyl 6 thioguanosine

MESG, a chromophoric substrate, can be used to quantitate inorganic phosphate.

1M6 (1-Methyl-6-nitroisatoic anhydride) is a SHAPE reagent for the detection of RNA chemical structures.

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.

7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) serves as a fluorescent probe for detecting hydrogen sulfide (H2S). It is characterized by a low detection limit (4×10^-6 mol L), high selectivity, and great sensitivity. At a concentration of 150 µg mL, it exhibits negligible cytotoxicity. The excitation wavelength is 331 nm, while in DMSO solvent, it emits at approximately 385 nm; upon addition of HS-, the fluorescence intensity significantly increases around 392 nm.

6, 8-Difluoro-7-hydroxy-4-methylcoumarin NHS ester is a molecule with an excitation wavelength of 365 nm and an emission wavelength of 460 nm. The NHS ester can be used to label primary amines (-NH2) on proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Ac-ANW-AMC is a fluorogenic substrate for the β5i LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released, and its fluorescence quantifies the activity of the β5i LMP7 subunit. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

TNP-ATP, a derivative of ATP, acts as an antagonist to the purinergic P2Y1, P2X3, and P2X2 3 receptors, demonstrating selectivity by showing lower inhibitory concentrations (IC50 = 6, 0.9, 7 nM, respectively) compared to the P2X2, P2X4, and P2X7 receptors (IC50 = 2, 15.2, >30 µM, respectively) in HEK293 cells expressing these human receptors. It effectively diminishes acetic acid-induced calcium influx in 1321N1 cells targeting P2X3 and P2X2 3 receptors (IC50 = 100 and 62 nM, respectively) and alleviates pain in a mouse model of visceral pain (ED50 = 6.35 µmol kg). Moreover, TNP-ATP exhibits a notable fluorescence characteristic, with an emission peak at 547 nm after excitation at 403 nm, which enhances four-fold and shifts to 538 nm when bound to the insulin-degrading enzyme (IDE).

Compound 1a, known as 1-(3',6'-Bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)-3-phenylurea, serves as a fast-responsive fluorescent probe for detecting gold ions in water and synthetic products [1].

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester (cDCFDASE) serves as a fluorescent probe for assessing the intracellular pH (pH in) in living cells under various external pH conditions (pH ex) [1]. This compound is utilized to measure pH dynamics within cells, facilitating critical insights into cellular function and behavior.

5(6)-Carboxy-2',7'-dichlorofluorescein serves as an excellent substrate for the MRP2 vesicular transport assay, offering superior detection and transport characteristics [1].

NBD-X acid is an environmentally sensitive fluorescent probe used to study biological polymers such as lipids and proteins, with a higher labelling yield than NBD and significantly reduced fluorescence intensity in aqueous solutions. NBD-X acid emits green to yellow fluorescence with excitation/absorption wavelengths of 465/535 nm.