5 ethynyl 2' deoxyuridine

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine used to monitor de novo DNA synthesis through click chemistry and serves as an alkyl chain-based PROTAC linker for synthesizing PROTACs.

5-Ethynyl-2'-deoxyuridine-13C2 (EdU-13C2) is a 13C-labeled version of 5-Ethynyl-2'-deoxyuridine. As a thymidine analog, 5-Ethynyl-2'-deoxyuridine (EdU) can be incorporated into replicating DNA molecules during cell proliferation. The conjugation reaction between EdU and a dye allows for efficient and rapid cell proliferation analysis. This compound also serves as a PROTAC linker, classified under alkyl chain types, and is used in the synthesis of PROTAC molecules. Additionally, 5-Ethynyl-2'-deoxyuridine functions as a click chemistry reagent, containing an alkyne group that can undergo a copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) with molecules that have an azide group.

5'-Ethynyl-2'-deoxycytidine (EdC) functions as a nucleoside analog effectively inhibiting the replication of herpes simplex virus-1 (HSV-1) KOS strain with an ID50 of 0.2 μg/mL. Additionally, it decreases virus-induced cytopathogenic effects in PRK cells for HSV-1, HSV-2, and vaccinia virus strains, demonstrating MIC values between 0.2 to 5 μg/mL. EdC acts as a thymidylate synthetase inhibitor, selectively impacting the DNA incorporation rates of [1',2'-3H]deoxyuridine compared to [CH3-3H]deoxythymidine in PRK cells with ID50s of 3 and 120 μg/mL, respectively. It further inhibits thymidine synthetase and curtails L1210 cell proliferation, a process reversible by deoxythymidine (ID50s are 4.4 and 1,000 μg/mL, respectively). Also, EdC is utilized in monitoring DNA synthesis and cellular replication through click chemistry conjugation, linking its ethynyl group with the azido group of various fluorochromes.