4-methylumbelliferyl-β-d-xylopyranoside

4-Methylumbelliferyl-β-D-xylopyranoside (4-Methylumbelliferyl-β-D-xyloside) exhibits bursting steady-state kinetics in biological experiments, which supports the conclusion of rapid hydrolysis of the glycosidic bond of the substrate 4-Methylumbelliferyl-β-D-xylopyranoside 4-Methylumbelliferyl-β-D-xylopyranoside can be used for β- xylosidase activity studies.

β-Xylosidase, Bacillus subtilis (EC 3.2.1.37), is capable of releasing reducing sugars from birchwood xylan and also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-xylopyranoside. This enzyme lacks endo-xylanase, arabinoxylanase, or β-glucanase activity. Furthermore, β-Xylosidase undergoes glycosylation modification after translation.