BODIPY 493/503 (Pyrromethene 546) is a lipophilic fluorescent probe with Ex/Em of 493/503 nm. BODIPY 493/503 localizes to polar lipids and can be used to label cellular neutral lipid contents and for live and fixed cell applications.
DAPI Dihydrochloride (FxCycle Violet) is A cell-permeable fluorescent probe. By binding to small forests rich in A-T DNA sequences, it is used to stain DNA and chromosomes, with a preference for DNA rich in adenine and thymine.
H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.
ER-Tracker Green (ERTG) is a cell permeable live cell stain for live cell imaging.ER-Tracker Green has an excitation wavelength of 504 nm and an emission wavelength of 511 nm.
Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm). Dihydrorhodamine 123 is oxidized to the fluorescent reagent Rhodamine 123 in the presence of intracellular reactive oxygen species and is localized in mitochondria.
FITC is a green fluorescein derivative. FITC is often used to label biological macromolecules such as antibodies and proteins. FITC can also be used to label nucleic acids. FITC can be used as a fluorescent probe to detect cell viability, cell apoptosis, etc. FITC showed a maximum excitation wavelength of 494 nm and yellow-green fluorescence at a maximum emission wavelength of 520 nm.
DiI (DiIC18(3)) is a lipophilic membrane dye commonly used as a long-term tracer for neurons and other cells. DiI fluoresces very weakly before it enters the cell membrane, and only after it enters the cell membrane can it be excited to emit a strong orange-red fluorescence (λex=549 nm, λem=565 nm).
C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.
MQAE, a fluorescent chloride ion indicator, is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. MQAE is quenched via collision with chloride.
Hoechst 33342 trihydrochloride (bisBenzimide H 33342 trihydrochloride;HOE 33342 trihydrochloride) is a membrane permeant DNA stain with blue fluorescence.
5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
Calcein-AM (Calcein acetoxymethyl ester) is a cell-permeable green fluorescent probe commonly used for assessing eukaryotic cell viability or mitochondrial permeability transition pore, with no fluorescence itself. After hydrolysis, it emits green fluorescence with excitation/emission wavelengths: 494/515 nm.
Calcein (Fluorexon) is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage, a complexometric indicator for titration of calcium ions with EDTA, and for the fluorometric determination of calcium.
Zinquin ethyl ester is a cell membrane-penetrating and selective fluorescent probe for zinc ions that measures intracellular levels of Zn(2+) and Ca(2+) ions and can be used to stain early embryos.