FITC-Dextran (MW 4000) is a fluorescein isothiocyanate (FITC)-labeled dextran fluorescent probe (Ex=495 nm; Em=525 nm) that combines the biocompatibility and hydrophilicity of dextran with the green fluorescence properties of FITC. FITC-Dextran (MW 4000) serves as a marker to reveal heat shock-induced cellular damage and investigate early and late stages of apoptosis. It is also applicable for studies on cellular permeability, such as blood-brain barrier permeability and assessment of blood-brain barrier disruption.
BODIPY 493/503 (Pyrromethene 546) is a lipophilic fluorescent probe with Ex/Em of 493/503 nm. BODIPY 493/503 localizes to polar lipids and can be used to label cellular neutral lipid contents and for live and fixed cell applications.
DAPI Dihydrochloride is a cell-permeable fluorescent probe. By binding to small forests rich in A-T DNA sequences, it is used to stain DNA and chromosomes, with a preference for DNA rich in adenine and thymine.
H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.
Hoechst 33342 (bisBenzimide H 33342) is a lipophilic fluorescent dye belonging to the bisbenzimidazole class and a cell-permeable fluorescent DNA dye. Hoechst 33342 can penetrate the membranes of living cells to achieve non-invasive staining of live cell nuclei, exhibiting excellent fluorescence properties and extremely low cytotoxicity.
Cyanine3 NHS Ester Chloride is an amine-reactive orange-red fluorescent dye and the NHS ester derivative of Cyanine3, supplied as a chloride salt. Its N-hydroxysuccinimide (NHS) ester reacts efficiently with primary amines on proteins, antibodies, or other biomolecules under neutral to slightly basic conditions, forming stable amide bonds. This dye is widely used for cellular labeling, flow cytometry, and biomolecular staining studies.
ER-Tracker Green (ERTG) is a cell permeable live cell stain for live cell imaging.ER-Tracker Green has an excitation wavelength of 504 nm and an emission wavelength of 511 nm.
D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays.
Dihydrorhodamine 123 (DHR 123) is a fluorescent probe specific for intracellular reactive oxygen species (ROS) (λex=488 nm, λem=525 nm), exhibiting high permeability to cell membranes and fluorescence activation upon oxidation. Dihydrorhodamine 123 is a classic tool for detecting cellular oxidative stress, reactive oxygen species (ROS) production, and antioxidant screening.
FITC is a green fluorescein derivative. FITC is commonly used to label biomolecules such as antibodies, proteins, and nucleic acids. It can serve as a fluorescent probe for detecting cell viability, apoptosis, and other cellular processes. FITC has a maximum excitation wavelength of 494 nm and emits yellow-green fluorescence at its maximum emission wavelength of 520 nm.
DiI (DiIC18(3)) is a lipophilic membrane dye commonly used as a long-term tracer for neurons and other cells. DiI fluoresces very weakly before it enters the cell membrane, and only after it enters the cell membrane can it be excited to emit a strong orange-red fluorescence (λex=549 nm, λem=565 nm).
C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.
FITC-Dextran (MW 60,000-76,000) is a fluorescein isothiocyanate (FITC)-labeled dextran fluorescent probe that is commonly used as a tracer molecule to label and track the action of cells or other biomaterials on semipermeable membranes.
MQAE, a fluorescent chloride ion indicator, is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. MQAE is quenched via collision with chloride.
Hoechst 33342 trihydrochloride (bisBenzimide H 33342 trihydrochloride;HOE 33342 trihydrochloride) is a membrane permeant DNA stain with blue fluorescence.
5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.