BODIPY 493/503 (Pyrromethene 546) is a lipophilic fluorescent probe with Ex/Em of 493/503 nm. BODIPY 493/503 localizes to polar lipids and can be used to label cellular neutral lipid contents and for live and fixed cell applications.
H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.
ER-Tracker Green (ERTG) is a cell permeable live cell stain for live cell imaging.ER-Tracker Green has an excitation wavelength of 504 nm and an emission wavelength of 511 nm.
D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays.
Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm). Dihydrorhodamine 123 is oxidized to the fluorescent reagent Rhodamine 123 in the presence of intracellular reactive oxygen species and is localized in mitochondria.
DiI (DiIC18(3)) is a lipophilic membrane dye commonly used as a long-term tracer for neurons and other cells. DiI fluoresces very weakly before it enters the cell membrane, and only after it enters the cell membrane can it be excited to emit a strong orange-red fluorescence (λex=549 nm, λem=565 nm).
C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.
FITC-Dextran (MW 60,000-76,000) is a fluorescein isothiocyanate (FITC)-labeled dextran fluorescent probe that is commonly used as a tracer molecule to label and track the action of cells or other biomaterials on semipermeable membranes.
MQAE, a fluorescent chloride ion indicator, is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. MQAE is quenched via collision with chloride.
Hoechst 33342 trihydrochloride (bisBenzimide H 33342 trihydrochloride;HOE 33342 trihydrochloride) is a membrane permeant DNA stain with blue fluorescence.
5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
Calcein-AM (Calcein acetoxymethyl ester) is a cell-permeable green fluorescent probe commonly used for assessing eukaryotic cell viability or mitochondrial permeability transition pore, with no fluorescence itself. After hydrolysis, it emits green fluorescence with excitation/emission wavelengths: 494/515 nm.
Calcein (Fluorexon) is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage, a complexometric indicator for titration of calcium ions with EDTA, and for the fluorometric determination of calcium.
Zinquin ethyl ester is a cell membrane-penetrating and selective fluorescent probe for zinc ions that measures intracellular levels of Zn(2+) and Ca(2+) ions and can be used to stain early embryos.
PE 154 is a potent fluorescent inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50s of 280 pM and 16 nM respectively. PE 154 is commonly used to label β-amyloid plaques in histochemical analysis.
BODIPY 558/568 C12 (Red C12) is an intracellular BODIPY dye that crosses cell membranes and is an orange-red fluorescent fatty acid that can be used to label living cells by localizing on intracellular neutral lipids for specific staining.
NBD-X acid is an environmentally sensitive fluorescent probe used to study biological polymers such as lipids and proteins, with a higher labelling yield than NBD and significantly reduced fluorescence intensity in aqueous solutions. NBD-X acid emits green to yellow fluorescence with excitation/absorption wavelengths of 465/535 nm.
Fura-2 AM is a membrane-permeable Ca²⁺ (calcium ion) indicator that is converted to Fura-2 by esterase within cells. Its fluorescence emission wavelength is around 510 nm, with excitation maxima at 340 nm under high calcium concentrations and 380 nm under low calcium concentrations. Calcium ion concentrations can be calculated using the fluorescence intensity ratio.