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Zinquin ethyl ester

Name: Zinquin ethyl ester
Brand: TargetMol
SKU: T78392
Price: 106 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T78392 Copy Product Info

Purity: 99.03%

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Zinquin ethyl ester is a cell membrane-penetrating and selective fluorescent probe for zinc ions that measures intracellular levels of Zn(2+) and Ca(2+) ions and can be used to stain early embryos.

Cas No. 181530-09-6

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Pack Size	Price	USA Stock	Global Stock
1 mg	$106	-	In Stock
5 mg	$326	-	In Stock
10 mg	$486	-	In Stock
25 mg	$779	-	In Stock
50 mg	$1,060	-	In Stock
100 mg	$1,430	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.03%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description

Zinquin ethyl ester is a cell membrane-penetrating and selective fluorescent probe for zinc ions that measures intracellular levels of Zn(2+) and Ca(2+) ions and can be used to stain early embryos.

Cell Research

RPE cell uptake of Zn2+ experiment
a. Solution preparation: Use DMSO to prepare a 10 mM Zinquin ethyl ester stock solution. For example, weigh 1 mg of Zinquin ethyl ester and add 0.4825 mL of DMSO.
Note: The prepared stock solution should be stored in aliquots to avoid repeated freezing and thawing. Unused stock solution should be divided into aliquots of single-use volume and stored at -20℃ in the dark.
b. Operation steps:
1. RPE cells were incubated with 0 ~ 18 nM Zn2+ in serum-free MEM without Zn2+ detection level for 60 minutes.
2. After removing extracellular Zn2+ from the culture, the cells were loaded with 2.5 mM zinc fluorescent sensor Zinquin ethyl ester for 30 minutes.
3. Observe the fluorescent Zinquin-Zn2+ complex under a fluorescence microscope with excitation at 364 nm and emission at 385 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	414.48
Formula	C₂₁H₂₂N₂O₅S
Cas No.	181530-09-6
Smiles	O=C(OCC)COC1=CC2=CC=C(N=C2C(=C1)NS(=O)(=O)C3=CC=C(C=C3)C)C

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 0.1 mg/mL (insoluble)

DMSO: 67.5 mg/mL (162.85 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 6.75 mg/mL (16.29 mM), Solution.

10% DMSO+90% Saline: < 6.75 mg/mL (16.29 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4127 mL	12.0633 mL	24.1266 mL	120.6331 mL
5 mM	0.4825 mL	2.4127 mL	4.8253 mL	24.1266 mL
10 mM	0.2413 mL	1.2063 mL	2.4127 mL	12.0633 mL
20 mM	0.1206 mL	0.6032 mL	1.2063 mL	6.0317 mL
50 mM	0.0483 mL	0.2413 mL	0.4825 mL	2.4127 mL
100 mM	0.0241 mL	0.1206 mL	0.2413 mL	1.2063 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Zinquin ethyl ester chemical structure | Zinquin ethyl ester formula | Zinquin ethyl ester molecular weight