Shopping Cart

Your shopping cart is currently empty

Hoechst 33342

Name: Hoechst 33342
Brand: TargetMol
SKU: T5840
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: HOE 33342, bisBenzimide H 33342) Copy Product Info

🥰Excellent

Hot

Synonyms: HOE 33342, bisBenzimide H 33342

Catalog No. T5840 Copy Product Info

Purity: 99.75%

🥰Excellent

Hot

Hoechst 33342 (bisBenzimide H 33342) is a lipophilic fluorescent dye belonging to the bisbenzimidazole class and a cell-permeable fluorescent DNA dye. Hoechst 33342 can penetrate the membranes of living cells to achieve non-invasive staining of live cell nuclei, exhibiting excellent fluorescence properties and extremely low cytotoxicity.

Cas No. 23491-52-3

Pack Size	Price	USA Stock	Global Stock
10 mg	$30	In Stock	In Stock
25 mg	$46	In Stock	In Stock
50 mg	$65	-	In Stock
100 mg	$92	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:99.75%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Hoechst 33342 AI Summary

Hoechst 33342 exhibits a wide range of bioactivities including DNA interactions, cytotoxicity against multiple cancer cell lines, antimicrobial properties, and effects on various transporters and enzymes. It demonstrates significant topoisomerase-1 mediated DNA cleavage (relative effective concentration: 1.0) and has binding affinity to calf thymus DNA with an equilibrium association constant (Ka) of 0.284 uM. Cytotoxicity is observed against several cancer cell lines: RPMI 8402 (IC50: 30.0 nM), CPT-K5 (IC50: 900.0 nM), KB3-1 (IC50: 10.0 nM), KBV-1 (IC50: 1200.0 nM), SK-Mel (IC50: 1600.0 nM), PC3 (IC50: 710.0 nM), U87 (IC50: 4200.0 nM), MCF7 (IC50: 500.0 nM), and HeLa (IC50: 300.0 nM). It also inhibits various microbial strains such as Escherichia coli, Staphylococcus aureus, and Candida species with MICs ranging from 0.125 to 16.0 ug/mL. Additionally, Hoechst 33342 modulates liver transporters OATP2B1, OATP1B3, and OATP1B1, and inhibits breast cancer resistance protein (BCRP) and ABCG2 in cellular assays. Its multifaceted bioactivities make it a potential candidate for further pharmacological and antimicrobial research..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Hoechst 33342 (bisBenzimide H 33342) is a lipophilic fluorescent dye belonging to the bisbenzimidazole class and a cell-permeable fluorescent DNA dye. Hoechst 33342 can penetrate the membranes of living cells to achieve non-invasive staining of live cell nuclei, exhibiting excellent fluorescence properties and extremely low cytotoxicity.
Targets & IC50	HEK293 cells:18 μM, HeLa cells:0.3 μM, A549 cells:0.98 μg/mL, U-87MG cells ATCC:4.2 μM, MCF-7 cells:0.5 μM, BEAS-2B cells:1.95 μg/mL, DU-145 cells:4.25 μM
In vitro	Methods: In vitro cultures of dorsal root ganglia (DRG) from Sprague Dawley rats on embryonic day 16 were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Hoechst 33342, PBS diluted 1:100, incubated at room temperature in the dark for 30 minutes, and observed under a fluorescence microscope (DAPI filter: 335-383/420-470 nm). Results: H33342 not only clearly labeled cell nuclei but also distinctly labeled DRG neurites with an excellent signal-to-noise ratio.[1]
Synonyms	HOE 33342, bisBenzimide H 33342

Chemical Properties

Molecular Weight	452.55
Formula	C₂₇H₂₈N₆O
Cas No.	23491-52-3
Smiles	CCOc1ccc(cc1)-c1nc2ccc(cc2[nH]1)-c1nc2ccc(cc2[nH]1)N1CCN(C)CC1
Relative Density.	1.274 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 41.7 mg/mL (92.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 0.5 mg/mL (1.1 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2097 mL	11.0485 mL	22.0970 mL	110.4850 mL
5 mM	0.4419 mL	2.2097 mL	4.4194 mL	22.0970 mL
10 mM	0.2210 mL	1.1049 mL	2.2097 mL	11.0485 mL
20 mM	0.1105 mL	0.5524 mL	1.1049 mL	5.5243 mL
50 mM	0.0442 mL	0.2210 mL	0.4419 mL	2.2097 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc