Lucifer Yellow CH dipotassium is a water-soluble fluorescent tracer that labels endocytosis vesicles in cells.Lucifer Yellow CH dipotassium excites and emits at a maximum wavelength of 430 nm and 540 nm, respectively.
Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.
Cyanine5 NHS ester chloride is a reactive dye with red fluorescence that requires a small amount of organic cosolvent (such as DMF or DMSO) to label amino groups in peptides, proteins, and oligonucleotides.
Hydroxystilbamidine ((E)-hydroxystilbamidine), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
BODIPY 630/650X is a fluorescently labeled conjugate of the adenosine receptor ligand N-ethylcarboxamido-adenosine (NECA), which exhibits distinct excitation and emission maxima at approximately 630 nanometers and 650 nanometers, respectively, making it a valuable tool for fluorescence-based assays and imaging studies targeting adenosine receptor activity and distribution.
DiSC3(5) is a lipophilic cationic fluorescent probe sensitive to changes in membrane potential. 3,3'-Dipropylthiadicarbocyanine iodide accumulates in hyperpolarized cell membranes, leading to fluorescence quenching; when the membranes are depolarized, the dye is released from the cells and the fluorescence intensity is enhanced, which is used for real-time monitoring of mitochondrial membrane potential changes and membrane hyperpolarization. The maximum excitation/emission wavelength was 622/670 nm.
DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Cyanine5.5 amine is an amino-modified Cy5.5 near-infrared (NIR) fluorescent dye with excitation/emission wavelengths of 648/710 nm. It offers the advantage of low fluorescence background and is suitable for group conjugation and in vivo imaging.
Cy5-DBCO is a near-infrared, anthocyanine fluorescent dye Cy5 (Cyanine 5) derivative with an excitation wavelength of 650 nm and an emission wavelength of 670 nm.DBCO-Sulfo-Cy5 contains a DBCO moiety, which can undergo SPAAC click chemistry with molecules that contain Azide moieties.
Alexa fluor 647 maleimide is a water-soluble fluorescent dye that maintains stability under a broad range of physiological conditions. Alexa fluor 647 maleimide facilitates the covalent attachment of fluorescent groups to proteins through selective reaction with thiol groups, thereby enabling precise labeling. This labeling method offers both high sensitivity and specificity, allowing detection of proteins at very low concentrations. Alexa fluor 647 maleimide is widely applied in the study of protein expression and localization, and it can also be conjugated to antibodies for labeling cell surface antigens or specific proteins within tissues [Ex/Em(nm)=651/671].
Cyanine5 amine, hydrochloride can undergo a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction with terminal alkynes and can also react with carboxyl groups to label polysaccharides. It has excitation/emission wavelengths of 646/662 nm.
Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derived from the Nile blue fluorophore, specifically designed for imaging thioredoxin (Trx) in live cells and in vivo, with excitation and emission wavelengths of 615 nm and 661 nm, respectively.
The rapid, selective, and sensitive sensing of thiols is important in diverse areas of research. This thiofluor 623 responds upon exposure to thiols with an increase in fluorescence intensity of up to 120-fold. The response is selective for thiols and occurs in aqueous media. In the absence of thiols, the probe is essentially non-fluorescent; thiols cause cleavage of the probe, generating a fluorophore with an absorption maximum of 563 nm and emission at 623 nm. The fluorescence quantum yield of the cleaved product, generated in response to thiols, increases in more viscous media, suggesting ideal performance in biological systems and applicability to single-molecule or 2-photon sensing schemes. The thiofluor 623 is cell-permeable and reacts selectively with intracellular thiols. The pseudo-first order rate constant (kobs) depends on substrate (e.g., 2.1 x 10-3 s-1 for cysteine, 2.0 x 10-5 s-1 for human serum albumin).
Di-4-ANEPPS, a voltage-sensitive dye, is utilized for detecting membrane potential changes, offering a time resolution under 1 ms and fluorescence variations up to 10% per 100 mV. Widely used for cardiac electrical activity mapping, Di-4-ANEPPS also shows a marked irreversible impact on both spontaneous heart rate and ventricular impulse conduction within an isolated rabbit heart model [1].