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C12FDG

Catalog No. T78393 CAS 138777-25-0

Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside

C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

C12FDG, CAS 138777-25-0

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1 mg	In stock	$ 299.00

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Purity: 99.84%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

Fluorescence aging-related β-galactosidase (SA-β-Gal) assay [1]:
1.Cultivate cells at a density of 10^5 cells/mL.
2.Wash cells once with 200 μL PBS, then fix with 100 μL fixation solution (2% formaldehyde/0.2% glutaraldehyde in1. distilled water) at room temperature for 5 minutes.
3.Wash cells twice with 200 μL PBS, stain with 100 μL 33 μM C12FDG (PBS, pH 6.0) for 10 minutes, then stain with 200 μL Hoechst solution (1 μg/mL Hoechst 33342) in PBS (pH 6.0) for 10 minutes.
4.Image cells using a 20x objective and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), monitor cells using emission filters of 460 nm and 535 nm.
This protocol is provided for reference and should be modified according to the specific requirements of your experiment.

Synonyms	5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
Molecular Weight	853.9
Formula	C44H55NO16
CAS No.	138777-25-0

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 30 mg/mL (35.13 mM), Sonication is recommended.

References and Literature

1. Udono M, Kadooka K, Yamashita S, Katakura Y. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8.

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Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

C12FDG 138777-25-0 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.