cytometry

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.

PKH 26 is a red-fluorescent membrane dye, Ex/Em=551/567 nm.PKH 26 is commonly used in flow cytometry to measure T cell-mediated cytotoxic activity.

GLF16 FA is a fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

Alexa fluor 647 NHS ester (L-5-Hydroxytryptophan-d3) is a photostable far-red fluorescent dye used in imaging and flow cytometry to generate stable signals for protein labeling, amine-modified oligonucleotides, and other primary amine-containing molecules.

BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.

GLF16 is a hydrophilic fluorescent SBB analog and robustly identifies senescence. It enables the use of flow cytometry and live cell sorting for rapid senescent cell detection. GLF16 is suitable for in vivo identification, isolation, and downstream characterization of senescent cell populations at the single-cell level in various biological materials.

C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.

Fluo-4 AM is a cell-permeable calcium ion (Ca²⁺) fluorescent indicator/chemical probe suitable for fluorescence imaging, flow cytometry, and plate screening, with an excitation wavelength of approximately 488 nm and an emission wavelength of 512–520 nm.

JC-1 (CBIC2) is a fluorescent lipophilic carbonyl cyanine dye. JC-1 is used to measure mitochondrial membrane potential. When the mitochondrial membrane potential is relatively high, JC-1 converges in the matrix to form a polymer, which can produce red fluorescence (λex=585 nm, λem=590 nm). When the mitochondrial membrane potential is low, JC-1 cannot accumulate in the mitochondrial matrix and exists in the form of monomers to produce green fluorescence (λex=510 nm, λem=527 nm).

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

CY7-SE is a CY7-derived dye with excitation/emission wavelengths of 750/775 nm. CY7 (Sulfo-Cyanine7) is an amine-reactive near-infrared (NIR) fluorophore with excitation/emission wavelengths of 745/800 nm, suitable for in vivo imaging, flow cytometry, antibody labelling, and nucleic acid detection, among other applications.

Fluo-3AM is a calcium indicator that exhibits an increase in fluorescence upon binding Ca2+. It is used to image the spatial dynamics of Ca2+ signaling in flow cytometry experiments.

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).

Galactose-PEG-Cy3 is a Cy3-labeled Galactose-PEG conjugate. The Cy3 fluorophore is frequently used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3's maximum emission wavelength ranges from approximately 562-570 nm. Galactose-PEG enhances cellular uptake of drugs and reduces endosomal degradation, making it valuable for drug delivery.

Chitosan-PEG-Cy3 is a fluorescent labeling reagent that combines the Cy3 fluorescent dye with Chitosan and polyethylene glycol (PEG). Cy3 fluorophores are frequently utilized in applications like immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The maximum emission wavelength of Cy3 is approximately 562-570 nm. Chitosan exhibits antimicrobial activity against various bacteria and fungi.

RGD-PEG-Cy3 is a fluorescent labeling reagent that combines the Cy3 fluorescent dye, peptide (RGD), and polyethylene glycol (PEG). The Cy3 fluorophore is commonly employed in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with a peak emission wavelength of approximately 562-570 nm. RGD is a peptide sequence (CRGDKGPDCiRGD) that targets tumor tissues specifically by binding to αvβ3 and αvβ5 integrin receptors, which are involved in tumor neovascularization.

TAT-PEG-Cy3 is a fluorescent labeling reagent that integrates the Cy3 fluorescent dye, a cell-penetrating peptide (TAT), and polyethylene glycol (PEG). The Cy3 fluorophore is commonly employed in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with a maximum emission wavelength of approximately 562–570 nm. TAT-PEG-Cy3 is suitable for cell-targeted delivery and bioimaging.

Dextran-CY3 is a fluorescent labeling reagent combining the Cy3 fluorescent dye with Dextran. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has a maximum emission wavelength of approximately 562-570 nm. Dextran inhibits platelet aggregation and clotting factors, and is used as a plasma volume expander.

Alexa Fluor 430 NHS ester is a compound used to conjugate the green fluorescent dye Alexa Fluor 430 with proteins or antibodies. Alexa Fluor 430 provides stable signal generation in imaging and flow cytometry. The NHS ester is suitable for labeling proteins, amine-modified oligonucleotides, and other primary amine-containing molecules (R-NH2).