cytometry

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.

Alexa fluor 647 NHS ester (L-5-Hydroxytryptophan-d3) is a photostable far-red fluorescent dye used in imaging and flow cytometry to generate stable signals for protein labeling, amine-modified oligonucleotides, and other primary amine-containing molecules.

BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.

GLF16 is a hydrophilic fluorescent SBB analog and robustly identifies senescence. It enables the use of flow cytometry and live cell sorting for rapid senescent cell detection. GLF16 is suitable for in vivo identification, isolation, and downstream characterization of senescent cell populations at the single-cell level in various biological materials.

GLF16 FA is a hydrophilic fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex Em of PI is 493 636 nm. Upon binding with nucleic acid, the Ex Em shifts to 535 617 nm, enhancing the fluorescence signal 20-30 times.

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

Fluo-3AM is a calcium indicator that exhibits an increase in fluorescence upon binding Ca2+. It is used to image the spatial dynamics of Ca2+ signaling in flow cytometry experiments.

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).

Galactose-PEG-Cy3 is a Cy3-labeled Galactose-PEG conjugate. The Cy3 fluorophore is frequently used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3's maximum emission wavelength ranges from approximately 562-570 nm. Galactose-PEG enhances cellular uptake of drugs and reduces endosomal degradation, making it valuable for drug delivery.

Chitosan-PEG-Cy3 is a fluorescent labeling reagent that combines the Cy3 fluorescent dye with Chitosan and polyethylene glycol (PEG). Cy3 fluorophores are frequently utilized in applications like immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The maximum emission wavelength of Cy3 is approximately 562-570 nm. Chitosan exhibits antimicrobial activity against various bacteria and fungi.

RGD-PEG-Cy3 is a fluorescent labeling reagent that combines the Cy3 fluorescent dye, peptide (RGD), and polyethylene glycol (PEG). The Cy3 fluorophore is commonly employed in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with a peak emission wavelength of approximately 562-570 nm. RGD is a peptide sequence (CRGDKGPDCiRGD) that targets tumor tissues specifically by binding to αvβ3 and αvβ5 integrin receptors, which are involved in tumor neovascularization.

TAT-PEG-Cy3 is a fluorescent labeling reagent that integrates the Cy3 fluorescent dye, a cell-penetrating peptide (TAT), and polyethylene glycol (PEG). The Cy3 fluorophore is commonly employed in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with a maximum emission wavelength of approximately 562–570 nm. TAT-PEG-Cy3 is suitable for cell-targeted delivery and bioimaging.

Dextran-CY3 is a fluorescent labeling reagent combining the Cy3 fluorescent dye with Dextran. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has a maximum emission wavelength of approximately 562-570 nm. Dextran inhibits platelet aggregation and clotting factors, and is used as a plasma volume expander.

Alexa Fluor 430 NHS ester is a compound used to conjugate the green fluorescent dye Alexa Fluor 430 with proteins or antibodies. Alexa Fluor 430 provides stable signal generation in imaging and flow cytometry. The NHS ester is suitable for labeling proteins, amine-modified oligonucleotides, and other primary amine-containing molecules (R-NH2).

eGFP mRNA-LNP (500 ng uL) is a lipid nanoparticle (LNP) containing eGFP mRNA, suitable for assays of RNA delivery, translation efficiency, and cell viability. eGFP circRNA includes the Enhanced Green Fluorescent Protein (eGFP), which expresses green fluorescent protein upon entering cells. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry.

Cy3-PEG-NH2 is a polyethylene glycol (PEG) derivative that incorporates a Cy3 fluorescent dye and an amino group. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Its maximum emission wavelength ranges from approximately 562 to 570 nm. Cy3-PEG-NH2 is applicable for fluorescence imaging, fluorescent tracing, and fluorescent labeling.

OVA-PEG-Cy3 is a conjugate of OVA-PEG labeled with Cy3. The Cy3 fluorophore is widely used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with its maximum emission wavelength approximately at 562-570 nm. Ovalbumins (OVA) are the primary proteins found in egg whites, known for their diverse biological activities, including anticancer, antihypertensive, antibacterial, antioxidant, and immunoregulatory effects. Ovalbumins are the most abundant proteins synthesized in oviducts treated with progesterone or estrogen and are commonly used as markers to study hormone regulation of gene expression in tissues.

DOX-PEG-Cy3 (Doxorubicin-PEG-Cy3) is a Cy3-labeled DOX-PEG conjugate. The Cy3 fluorophore is commonly used for applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The maximum emission wavelength of Cy3 is approximately 562-570 nm. DOX is a broad-spectrum anthracycline antibiotic with cytotoxic properties.