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BODIPY FL prazosin

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TargetMol
Catalog No. T63980Cas No. 175799-93-6

BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.

BODIPY FL prazosin

BODIPY FL prazosin

🥰Excellent
TargetMol
Purity: 97.27%
Catalog No. T63980Cas No. 175799-93-6
BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.
Pack SizePriceAvailabilityQuantity
1 mg$293In Stock
5 mg$722In Stock
10 mg$987In Stock
25 mg$1,520In Stock
50 mg$1,980In Stock
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Purity:97.27%
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Product Introduction

Bioactivity
Description
BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.
Targets&IC50
α1B-adrenergic receptor:43.3 nM (Ki), α1A-adrenergic receptor:14.5 nM (Ki)
In vitro
BODIPY FL prazosin (10 nM; 30 min at room temperature in 100 μl; COS-7 cells) shows Affinity of various α1-AR ligands with K i values of 14.5, 43.3 nM for α1a-AR and α1b-AR, respectively [1]. BODIPY FL prazosin (100 nM, 30 min) can be used as molecular probe for the Visualization of the non-adrenoceptor binding site of α1-adrenergic drugs in erythroleukemia cells [3].
Cell Research
Instructions for use
I. Solution preparation
1. Mother solution dissolution and preparation: BODIPY FL prazosin is recommended to be dissolved in anhydrous DMSO. The concentration can be adjusted according to the experimental requirements, usually between 10-50 μM.
2. Preparation of working solution: It can be diluted to the required concentration with buffer or culture medium according to the experimental needs. Usually, a certain preliminary experiment can be performed before use to optimize the final concentration.
II. Operation steps
1. Labeling reaction:
1) Add BODIPY FL prazosin solution (1-10uM) to the cell culture system. Generally, it is cultured at 37°C for a certain period of time (30 minutes to 1 hour) to ensure that the fluorescent label binds to the α1-AR on the cell surface.
2) During the labeling process, it should be ensured that the labeling agent does not have a toxic effect on the cells. It is recommended to use appropriate control experiments.
2. Cleaning and analysis:
1) After labeling, use an appropriate washing buffer to remove unbound fluorescent ligands to reduce background signals.
2) When performing flow cytometry or confocal microscopy analysis, ensure that the excitation light source and filter settings are correct to accurately collect fluorescence signals in the 485/535 nm wavelength range.
3. Analysis:
1) Use flow cytometry for quantitative analysis and evaluate the expression level of α1-AR by intracellular fluorescence intensity.
2) When using confocal microscopy, fluorescently labeled cells can be observed at high resolution to study the distribution and localization of receptor subtypes.
Notes:
1. BODIPY FL prazosin should be stored at -20°C to avoid repeated freezing and thawing.
2. The solution used in the experiment should be as fresh as possible and avoid long-term exposure to strong light to maintain its fluorescence stability.
Chemical Properties
Molecular Weight563.41
FormulaC28H32BF2N7O3
Cas No.175799-93-6
SmilesO=C(N1CCN(C=2N=C(N)C=3C=C(OC)C(OC)=CC3N2)CC1)CCC4=CC=C5C=C6C(=CC(=[N]6[B+3]([F-])([F-])[N-]54)C)C
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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