fluorogenic

hCYP3A4 Fluorogenic substrate 1, a phthalimide derivative, exhibits low activity against COX.

MMP-3 Fluorogenic Substrate, a compound designed for matrix metalloproteinase-3 (MMP-3) activity quantification, releases 7-methoxycoumarin-4-acetyl (Mca) upon MMP-3 cleavage. Mca's fluorescence, with excitation and emission maxima at 328 and 420 nm respectively, serves as a measurable indicator of MMP-3 activity.

Calpain-1 substrate, fluorogenic, is a sensitive and specific substrate for calpain-1 that cleaves the Tyr-Gly bond, resulting in enhanced fluorescence [1] (Ex Em = 490 nm 518 nm).

SGPL1 fluorogenic substrate is a fluorogenic substrate of sphingosine-1-phosphate lyase (SGPL1) used to measure SGPL1 activity [1].

4-Methylumbelliferyl phosphate (4-MUP) (4-MUP) is used as a fluorogenic substrate of alkaline phosphatases.

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrate (λex=362 nm, λem=445 nm).

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

CUG is a fluorogenic substrate (λex=386, λem=445 nm, ε=32K).

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).

PL553 is a specific and high-affinity fluorogenic substrate of Leukotriene A4 hydrolase [λmax: 210 nm; λem: 410 nm].

Janelia Fluor 525, Haloalkane is a yellow, cell-permeable, fluorogenic fluorescent dye featuring a chloroalkane handle that exhibits a high degree of fluorogenicity. The excitation and emission maxima (λ) of this compound are 525 nm and 549 nm, respectively, with a quantum yield of 0.91.

Janelia Fluor 549, Haloalkane, a yellow cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits a high level of fluorogenicity. The compound's excitation and emission maxima (λ) are respectively 549 nm and 571 nm, with a quantum yield = 0.88.

Janelia Fluor 585, Haloalkane, is an orange cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle. This compound exhibits a high level of fluorogenicity. It has excitation and emission maxima (λ) at 585 nm and 609 nm, respectively, with a quantum yield of 0.78.

Janelia Fluor 635, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 635 nm and 652 nm, respectively, with a quantum yield of 0.56.

Janelia Fluor 646, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 646 nm and 664 nm, respectively, with a quantum yield of 0.54.

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation emission maxima of 405 450 nm, respectively.

CytoCalcein Violet 500 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 500 is retained by living cells and displays excitation emission maxima of 405 500 nm, respectively.

Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation emission maxima of 525 550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

Calcein Red AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Red AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Red by intracellular esterases. Calcein Red is retained by living cells and displays excitation emission maxima of 560 574 nm, respectively. Calcein Red AM is a red-emitting variant of the green fluorophore calcein AM , which has been used to evaluate cell viability and cytotoxicity.

Ac-DNLD-AMC is a fluorogenic caspase-3 substrate. Upon enzymatic cleavage by caspase-3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-3 activity. AMC displays excitation emission maxima of 340-360 and 440-460 nm, respectively.

STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016). STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3 References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016).

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation emission maxima of 380 515 nm, respectively.1 1.Imai, K., Toyo'oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

Mca-PLAC(p-OMeBz)-WAR(Dpa)-NH2 is a fluorogenic substrate for matrix metalloproteinase-14 (MMP-14). Upon cleavage by MMP-14, 7-methoxycoumarin-4-acetyl (Mca) is released and its fluorescence can be used to quantify MMP activity. Mca displays excitation emission maxima of 328 420 nm, respectively.