fluorogenic

MMP-3 Fluorogenic Substrate, a compound designed for matrix metalloproteinase-3 (MMP-3) activity quantification, releases 7-methoxycoumarin-4-acetyl (Mca) upon MMP-3 cleavage. Mca's fluorescence, with excitation and emission maxima at 328 and 420 nm respectively, serves as a measurable indicator of MMP-3 activity.

SGPL1 fluorogenic substrate is a fluorogenic substrate of sphingosine-1-phosphate lyase (SGPL1) used to measure SGPL1 activity [1].

4-Methylumbelliferyl phosphate (4-MUP) (4-MUP) is used as a fluorogenic substrate of alkaline phosphatases.

Z-Ala-Arg-Arg-AMC (Fluorogenic Proteasome Substrate) is a fluorogenic substrate utilized to assay trypsin-like activity within the proteasome [1].

EDANS (EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).

α-Secretase Substrate II, Fluorogenic is an internally quenched fluorescent peptide substrate for α-secretase, featuring the α-secretase cleavage site of β-amyloid precursor protein (APP). Its excitation/emission (Ex/Em) wavelengths are 340/490 nm.

hCYP3A4 Fluorogenic substrate 1, a phthalimide derivative, exhibits low activity against COX.

Calpain-1 substrate, fluorogenic, is a sensitive and specific substrate for calpain-1 that cleaves the Tyr-Gly bond, resulting in enhanced fluorescence [1] (Ex/Em = 490 nm/518 nm).

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrate (λex=362 nm, λem=445 nm).

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

CUG is a fluorogenic substrate (λex=386, λem=445 nm, ε=32K).

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).

PL553 is a specific and high-affinity fluorogenic substrate of Leukotriene A4 hydrolase [λmax: 210 nm; λem: 410 nm].

Janelia Fluor 525, Haloalkane is a yellow, cell-permeable, fluorogenic fluorescent dye featuring a chloroalkane handle that exhibits a high degree of fluorogenicity. The excitation and emission maxima (λ) of this compound are 525 nm and 549 nm, respectively, with a quantum yield of 0.91.

Janelia Fluor 549, Haloalkane, a yellow cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits a high level of fluorogenicity. The compound's excitation and emission maxima (λ) are respectively 549 nm and 571 nm, with a quantum yield = 0.88.

Janelia Fluor 585, Haloalkane, is an orange cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle. This compound exhibits a high level of fluorogenicity. It has excitation and emission maxima (λ) at 585 nm and 609 nm, respectively, with a quantum yield of 0.78.

Janelia Fluor 635, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 635 nm and 652 nm, respectively, with a quantum yield of 0.56.

Janelia Fluor 646, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 646 nm and 664 nm, respectively, with a quantum yield of 0.54.

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation/emission maxima of 405/450 nm, respectively.

CytoCalcein Violet 500 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 500 is retained by living cells and displays excitation/emission maxima of 405/500 nm, respectively.

Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation/emission maxima of 525/550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

Calcein Red AM, a red-emitting derivative of the green fluorophore calcein AM, is a cell-permeable, non-fluorescent acetoxymethyl ester compound commonly utilized to assess cell viability and cytotoxicity. following intracellular esterase cleavage, the fluorescent product is retained in live cells and exhibits characteristic excitation and emission peaks at 560 nm and 574 nm, respectively.

Ac-DNLD-AMC is a fluorogenic caspase-3 substrate. Upon enzymatic cleavage by caspase-3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-3 activity. AMC displays excitation/emission maxima of 340-360 and 440-460 nm, respectively.