4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrate (λex=362 nm, λem=445 nm).

Bacterial deglucuronidation activity assay
I. Prepare bacterial lysate.
II. Operation steps
1. Dilute the bacterial lysate with reaction buffer (containing 1x protease inhibitor), transfer to a transparent 96-well plate, and pre-incubate at 37°C with shaking at 250 rpm for 10 minutes;
2. The final reaction conditions are 1 μg of bacterial isolated lysate protein, 200 μM 4-MUG added to the reaction buffer, and the final volume is 100 μL;
3. Incubate at 250 rpm at 37°C for 24 hours;
4. Determine the formation of 4-MU by fluorescence measurement at specific time points, with an excitation wavelength of 330 nm and an emission wavelength of 450 nm.