4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrate (λex=362 nm, λem=445 nm).

Bacterial deglucuronidation activity assay
I. Prepare bacterial lysate.
II. Operation steps
1. Dilute the bacterial lysate with reaction buffer (containing 1x protease inhibitor), transfer to a transparent 96-well plate, and pre-incubate at 37°C with shaking at 250 rpm for 10 minutes;
2. The final reaction conditions are 1 μg of bacterial isolated lysate protein, 200 μM 4-MUG added to the reaction buffer, and the final volume is 100 μL;
3. Incubate at 250 rpm at 37°C for 24 hours;
4. Determine the formation of 4-MU by fluorescence measurement at specific time points, with an excitation wavelength of 330 nm and an emission wavelength of 450 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 150 mg/mL (405.07 mM), Sonication is recommended.

H2O: 5.2 mg/mL (14.04 mM), Sonication and heating are recommended.