Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

MUF-diNAG

Name: MUF-diNAG
Brand: TargetMol
SKU: T37567
Rating: 4.5 (1 reviews)

Copy Product Info

😃Good

Catalog No. T37567Cas No. 53643-12-2

Alias 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobioside

MUF-diNAG (4-μU-(GlcNAc)2) is a fluorogenic substrate specifically designed for chitinases and chitobiosidases, which enzymatically cleave the molecule to release the fluorescent moiety 4-μU, MUF-diNAG exhibit pH-dependent excitation maxima at 320 and 360 nm and an emission maximum between 445 and 455 nm, thereby enabling sensitive and quantitative analysis of chitinolytic enzyme activity in biochemical and microbiological assays.

MUF-diNAG

Copy Product Info

😃Good

Catalog No. T37567Alias 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobiosideCas No. 53643-12-2

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	$497	35 days	35 days

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Appearance:Solid

Color:White

Resource Download

COA

Product Introduction

Bioactivity

Description	MUF-diNAG (4-μU-(GlcNAc)2) is a fluorogenic substrate specifically designed for chitinases and chitobiosidases, which enzymatically cleave the molecule to release the fluorescent moiety 4-μU, MUF-diNAG exhibit pH-dependent excitation maxima at 320 and 360 nm and an emission maximum between 445 and 455 nm, thereby enabling sensitive and quantitative analysis of chitinolytic enzyme activity in biochemical and microbiological assays.
In vitro	MUF-diNAG functions as a fluorogenic substrate for chitinases, including human Chitotriosidase (CHIT1). The enzyme recognizes the N,N'-diacetylchitobiose moiety and cleaves the β-glycosidic bond between the sugar chain and the 4-MU group. While the intact substrate is non-fluorescent, hydrolysis releases free 4-Methylumbelliferone, which emits blue fluorescence at 450 nm when excited at 360 nm [2].
In vivo	In models of Gaucher disease, the hydrolysis of MUF-diNAG in plasma serves as a biochemical marker for the total burden of lipid-laden macrophages. Furthermore, CHIT1 is secreted by activated macrophages during immune responses; thus, the substrate is employed to assess the severity of inflammation in conditions such as sarcoidosis, malaria, and fungal infections [1][2].
Synonyms	4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobioside

Chemical Properties

Molecular Weight	582.55
Formula	C₂₆H₃₄N₂O₁₃
Cas No.	53643-12-2
Smiles	O([C@@H]1[C@@H](CO)O[C@@H](OC=2C=C3C(=CC2)C(C)=CC(=O)O3)[C@H](NC(C)=O)[C@H]1O)[C@H]4[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O4

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMF: 10 mg/mL (17.17 mM), Sonication is recommended.

DMSO: 10 mg/mL (17.17 mM), Sonication is recommended.

Solution Preparation Table

DMF/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7166 mL	8.5830 mL	17.1659 mL	85.8295 mL
5 mM	0.3433 mL	1.7166 mL	3.4332 mL	17.1659 mL
10 mM	0.1717 mL	0.8583 mL	1.7166 mL	8.5830 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

4Methylumbelliferyl βDN,N'diacetylchitobioside4 Methylumbelliferyl β D N,N' diacetylchitobioside