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MUF-diNAG

Name: MUF-diNAG
Brand: TargetMol
SKU: T37567
Rating: 4.5 (1 reviews)

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Catalog No. T37567Cas No. 53643-12-2

Alias 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobioside

MUF-diNAG (4-μU-(GlcNAc)2) is a fluorogenic substrate specifically designed for chitinases and chitobiosidases, which enzymatically cleave the molecule to release the fluorescent moiety 4-μU, MUF-diNAG exhibit pH-dependent excitation maxima at 320 and 360 nm and an emission maximum between 445 and 455 nm, thereby enabling sensitive and quantitative analysis of chitinolytic enzyme activity in biochemical and microbiological assays.

MUF-diNAG

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Catalog No. T37567Alias 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobiosideCas No. 53643-12-2

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	$497	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	MUF-diNAG (4-μU-(GlcNAc)2) is a fluorogenic substrate specifically designed for chitinases and chitobiosidases, which enzymatically cleave the molecule to release the fluorescent moiety 4-μU, MUF-diNAG exhibit pH-dependent excitation maxima at 320 and 360 nm and an emission maximum between 445 and 455 nm, thereby enabling sensitive and quantitative analysis of chitinolytic enzyme activity in biochemical and microbiological assays.
In vitro	MUF-diNAG functions as a fluorogenic substrate for chitinases, including human Chitotriosidase (CHIT1). The enzyme recognizes the N,N'-diacetylchitobiose moiety and cleaves the β-glycosidic bond between the sugar chain and the 4-MU group. While the intact substrate is non-fluorescent, hydrolysis releases free 4-Methylumbelliferone, which emits blue fluorescence at 450 nm when excited at 360 nm [2].
In vivo	In models of Gaucher disease, the hydrolysis of MUF-diNAG in plasma serves as a biochemical marker for the total burden of lipid-laden macrophages. Furthermore, CHIT1 is secreted by activated macrophages during immune responses; thus, the substrate is employed to assess the severity of inflammation in conditions such as sarcoidosis, malaria, and fungal infections [1][2].
Synonyms	4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside, 4-Methylumbelliferyl N,N-diacetyl-β-D-chitobioside

Chemical Properties

Molecular Weight	582.55
Formula	C₂₆H₃₄N₂O₁₃
Cas No.	53643-12-2
Smiles	O([C@@H]1[C@@H](CO)O[C@@H](OC=2C=C3C(=CC2)C(C)=CC(=O)O3)[C@H](NC(C)=O)[C@H]1O)[C@H]4[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O4
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year

Solubility Information

DMF: 10 mg/mL (17.17 mM), Sonication is recommended.

DMSO: 10 mg/mL (17.17 mM), Sonication is recommended.

Solution Preparation Table

DMF/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7166 mL	8.5830 mL	17.1659 mL	85.8295 mL
5 mM	0.3433 mL	1.7166 mL	3.4332 mL	17.1659 mL
10 mM	0.1717 mL	0.8583 mL	1.7166 mL	8.5830 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline /PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline /PBS/ddH₂O TargetMol | reagent

mix well and clarify

The above example illustrates how to use "In Vivo Formulation Calculator" and does not represent a recommended formulation for any specific compound. Please select an appropriate dissolution method based on your experimental animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

4Methylumbelliferyl βDN,N'diacetylchitobioside4 Methylumbelliferyl β D N,N' diacetylchitobioside