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AC-DEVD-AFC

Name: AC-DEVD-AFC
Brand: TargetMol
SKU: TP1326
Price: 47 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TP1326Cas No. 201608-14-2

AC-DEVD-AFC, a fluorogenic substrate, undergoes cleavage by caspase-3, producing a blue fluorescent compound upon excitation at 400 nm, which can be quantified at an emission maximum of 505 nm.

AC-DEVD-AFC

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Purity: 99.11%

Catalog No. TP1326Cas No. 201608-14-2

AC-DEVD-AFC, a fluorogenic substrate, undergoes cleavage by caspase-3, producing a blue fluorescent compound upon excitation at 400 nm, which can be quantified at an emission maximum of 505 nm.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$47	In Stock	In Stock
5 mg	$147	In Stock	In Stock
10 mg	$277	In Stock	In Stock
25 mg	$469	In Stock	In Stock
50 mg	$675	In Stock	In Stock
100 mg	$942	-	In Stock
1 mL x 10 mM (in DMSO)	$258	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.11%

Appearance:Solid

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description

AC-DEVD-AFC, a fluorogenic substrate, undergoes cleavage by caspase-3, producing a blue fluorescent compound upon excitation at 400 nm, which can be quantified at an emission maximum of 505 nm.

In vitro

Caspase activation was measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC). We show that this substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-amino-4-trifluoromethyl coumarin (AFC). Following cell disruption, released AFC was separated on HPLC and detected by fluorescence. The appearance of AFC in cells was blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases were responsible for the cleavage. Caspase activity was first noted after about 2 h of incubation with doxorubicin or dactinomycin, the production of AFC being linear with time afterward[1].

Chemical Properties

Molecular Weight	729.61
Formula	C₃₀H₃₄F₃N₅O₁₃
Cas No.	201608-14-2
Smiles	CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F
Relative Density.	1.495 g/cm3
Sequence	N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoroMethylcoumarin
Sequence Short	DEVD-7-amido-4-trifluoroMethylcoumarin

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (54.82 mM), Sonication is recommended.

H2O: insoluble

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (2.74 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3706 mL	6.8530 mL	13.7060 mL	68.5298 mL
5 mM	0.2741 mL	1.3706 mL	2.7412 mL	13.7060 mL
10 mM	0.1371 mL	0.6853 mL	1.3706 mL	6.8530 mL
20 mM	0.0685 mL	0.3426 mL	0.6853 mL	3.4265 mL
50 mM	0.0274 mL	0.1371 mL	0.2741 mL	1.3706 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc