DAPI Dihydrochloride (FxCycle Violet) is a cell-permeable fluorescent probe by binding in the minor grove of A-T rich sequences of DNA, used to stain DNA and chromosomes, with a preference for adenine and thymine rich DNA.
MQAE, a fluorescent chloride ion indicator, is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. MQAE is quenched via collision with chloride.
Hoechst 33342 (bisBenzimide H 33342), is a Benzimidazole fluorescent dye and a Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. HOE 33342 trihydrochlorde is used to quantify DNA in viable cells.
Bromobimane (Monobromobimane) is a non-fluorescent substance, but reacts with thiols to produce a fluorescent product.Bromobimane is used as a probe, often in clinical medicine, to test for sulfide levels in blood.
Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.
Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication. it also interferes with mitosis.
ABMDMA (9,10-Anthracenediyl-bis(methylene)dimalonic acid) is a chemical probe and indicator with photobleaching properties that can be photobleached by mono-linear oxygen to the corresponding peroxylactone, and can be used to determine photo-induced mono-linear oxygen generation (SOG).
7-Hydroxycoumarin-3-carboxylic acid (7-HC-3-CA) is a safe anti-browning agent that inhibits tyrosinase activity, used in food preservation and human pigmentation research.
2,3-Diaminonaphthalene is a highly selective fluorometric and colorimetric reagent for selenium detection, and it is also used for the fluorometric determination of nitrite.
Msr-Ratio (Msr-green) (Msr-green) is a ratiometric fluorescent probe specifically designed for the detection of methionine sulfoxide reductase (MSR) activity. It has an excitation wavelength of 375 nm and an emission wavelength of 550 nm. This probe offers the ability to monitor the enzyme activity both in vitro and in live cells, making it a valuable tool for studying MSR function.
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. HOE 33187 is a cell dye for DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid) is an amine-reactive building block recognized by anti-DNP antibodies and serves as an excellent amine-reactive FRET quencher when paired with Trp or Tyr.
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is an amine-reactive building block recognized by anti-DNP antibodies and serves as an excellent amine-reactive FRET quencher when paired with Trp or Tyr.
HOE-S 785026 trihydrochloride is a live cell labeling dye that binds to nucleic acids by targeting the minor groove in DNA double strands, with a preference for A T-rich regions (fluorescence is significantly enhanced by A T-rich double-stranded DNA). It crosses cell membranes and binds to living or immobilized cells, with its fluorescence intensity increasing as the solution pH rises.
7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is a useful organic compound for research related to life sciences. The catalog number is T67683 and the CAS number is 96735-88-5.
Z-Arg-Arg-4MβNA triacetate serves as a specific substrate for cathepsin B, producing the fluorescent product 4MβNA, which has an excitation wavelength (λ ex) of 355 nm and an emission wavelength (λ em) of 430 nm [1] [2].
Phalloidin-AMCA conjugate is a blue fluorophore that specifically labels filamentous actin (F-actin). It consists of aminomethylcoumarin (AMCA; ex em max = 350 450 nm) conjugated with phalloidin , a mycotoxin that binds F-actin. Phalloidin-AMCA conjugate is used in fixed and permeabilized tissue sections, cell cultures, or cell-free experiments.
DPPP is a probe that reacts stoichiometrically with hydroperoxides to yield the fluorescent molecule diphenyl-1-pyrenylphosphine oxide (DPPP-O). Plasma levels of lipid hydroperoxides of phosphatidylcholine, phosphatidylethanolamine, triglycerides, and cholesteryl esters have been measured by HPLC with a post column detection system using DPPP. DPPP has also been used as a fluorescent probe for the detection of low density lipoprotein and cellular oxidation. Fluorescence of DPPP-O can be monitor using excitation and emission wavelengths of 351 nm and 380 nm, respectively.
2-(7-Methoxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64951 and the CAS number is 62935-72-2.
4-Methylumbelliferyl phosphate disodium (4-MUP), an anionic organophosphate, serves as a fluorogenic substrate for acid and alkaline phosphatases. Additionally, this compound acts as a simulant for nerve agents [1] [2] [3].
Bz-FVR-AMC is a fluorogenic substrate utilized for procathepsin, demonstrating a catalytic efficiency (k_cat K_m) of 1070 mM^-1 s^-1. Notably, elevated levels of Bz-FVR-AMC can inhibit the substrate [1][2]. For storage, it is essential to protect this compound from light exposure.
Z-DEVD-AMC is a selective substrate for caspase-3, measurable through fluorescence spectrometry, and serves as a fluorescence reference standard for AMC-based enzyme substrates, including those for caspase [1].
Indo-1 (potassium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (potassium salt) binds calcium. Indo-1 (potassium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.
Ac-EEVVAC-pNA serves as a chromogenic substrate specifically designed for the continuous spectrophotometric assay of the HCV NS3 protease. Its sequence, EEVVAC, originates from the 5A-5B cleavage junction within the HCV polyprotein [1].
The amine-reactive AMCA, SE (7-amino-4-methylcoumarin-3-acetic acid, succinimidyl ester) and its conjugates yield blue-fluorescence (approximate excitation emission maxima ~353 442) that can be used as a contrasting color in multicolor applications.