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ortho-iodoHoechst 33258

Name: ortho-iodoHoechst 33258
Brand: TargetMol
SKU: T19043
Rating: 4.5 (1 reviews)

Catalog No. T19043 Copy Product Info

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ortho-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation.

ortho-iodoHoechst 33258

Copy Product Info

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Catalog No. T19043

ortho-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation.

Cas No. 158013-41-3

Pack Size	Price	USA Stock	Global Stock
5 mg	$347	7-10 days	7-10 days
10 mg	$541	7-10 days	7-10 days
1 mL x 10 mM (in DMSO)	$397	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description

ortho-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation.

Chemical Properties

Molecular Weight	534.39
Formula	C₂₅H₂₃IN₆
Cas No.	158013-41-3
Smiles	CN1CCN(CC1)c1ccc2nc([nH]c2c1)-c1ccc2nc([nH]c2c1)-c1ccccc1I
Relative Density.	1.587 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (93.56 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (4.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8713 mL	9.3565 mL	18.7129 mL	93.5646 mL
5 mM	0.3743 mL	1.8713 mL	3.7426 mL	18.7129 mL
10 mM	0.1871 mL	0.9356 mL	1.8713 mL	9.3565 mL
20 mM	0.0936 mL	0.4678 mL	0.9356 mL	4.6782 mL
50 mM	0.0374 mL	0.1871 mL	0.3743 mL	1.8713 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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