CY7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm). Cy7 is used for labeling oligonucleotides, antibodies, peptides, and proteins.
DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation emission maxima of 646 662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Cyanine7 NHS ester is a fluorescent probe widely utilized in biomarking and bioimaging applications. It has excitation emission wavelengths (Ex Em) of 740 770 nm.
Cyanine5 amine, hydrochloride can undergo a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction with terminal alkynes and can also react with carboxyl groups to label polysaccharides. It has excitation emission wavelengths of 646 662 nm.
CY7-SE (Sulfo-Cyanine7 Succinimidyl Ester) is a fluorescence labeling agent (Ex=700-770 nm,Em=790 nm) used for labeling proteins, peptides, antibodies, and oligonucleotides.
DIBA-Cy5, a fluorescent DIBA antagonist, consists of DIBA-alkyne and Cyanine5 fluorophores (Cy5) linked by a polyethylene glycol (PEG) chain. It functions as a fluorescent ligand for probe attachment via click chemistry, exhibiting high binding affinity to type-2 mAChR (M2R) with a dissociation constant (Kd) of 1.80 nM. Notably, DIBA-Cy5 effectively stains M2R receptors in the sinoatrial node of mouse hearts [1].
Cyanine5-C6-DBCO is a low-toxicity azide-reactive probe (near-infrared fluorescent dye) used for imaging azide-labeled biomolecules via a copper-free click reaction. Its excitation emission wavelengths are 647 668 nm.
CY5-DBC0 is utilized in cellular imaging to label cell membranes, nuclei, or other organelles, thus revealing cell morphology, structure, and function. In tissue imaging, it helps observe changes in the structure and function of deep tissues like blood vessels. Additionally, CY5-DBC0 is applied in fields such as protein labeling, drug delivery, and biosensing, providing tools and methodologies for biomedical research. The excitation emission (Ex Em) wavelengths are 640 663 nm.
Cyanine7 amine is effective for labeling biomolecules such as proteins, nucleic acids, and cells, facilitating localization, tracking, and detection in various bioanalytical techniques. It provides fluorescent signals for visualization. The excitation emission wavelengths (Ex Em) are 750 773 nm.
AF647 C2 maleimide is a water-soluble fluorescent dye that remains stable under a range of physiological conditions. It facilitates the attachment of fluorescent groups to proteins by reacting with thiol groups on target proteins. This labeling method offers high sensitivity and specificity, enabling the detection of proteins at very low concentrations, making it valuable for studying protein expression and localization. Additionally, it can bind to antibodies for labeling cell surface antigens or specific proteins within tissues. [Ex Em(nm)=651 671].
Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, acts as a reporter fluorescent substrate in horseradish peroxidase (HRP)-catalyzed deposition, a signal amplification method used in immunoassays and in situ hybridization of nucleic acids.
BODIPY 650-X, SE is characterized by an excellent molar extinction coefficient and emission quantum yield. The NHS ester (N-hydroxysuccinimide ester) component allows it to couple with amino groups on proteins, peptides, and other molecules. This compound includes an additional aminohexanoic acid linker. [Ex Em(nm)=646 660].
AF647 carboxylic acid is a dye from the AF dye family that emits far-red fluorescence, minimizing spectral overlap and enhancing resolution in multicolor fluorescence experiments. It has a high quantum yield, producing strong fluorescence signals suitable for high-sensitivity detection and analysis. With high solubility in water and most organic solvents, its Ex Em (nm) is 648 671.